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[Determination of ilexside II in leaves of Ilex cornuta by HPLC-ELSD]
Zhimrong Yao1, Jun Li, Mingbo Zhao
1State Key Laboratory of Natural and Biomimetic Drugs, School of Phannrmaceutical Sciences, Peking University, Beijing 100191, China.
Objective:
To develop a HPLC-ELSD method for determination of ilexside II in leaves of Ilex cornuta.
Method:
The separation was performed on a Waters Syemmetry Shield RP18 column (4.6 mm x 250 mm, 5 microm) and methanol-water was used as mobile phase in gradient elution. The flow was 1.0 mL x min(-1), and the temperature was set at 35 degrees C.
Result:
The calibration curve showed good linearity in the test range (R2 = 0.9997). And the mean recovery was 101.3%, RSD was 2.0% (n=6).
Conclusion:
The developed HPLC-ELSD method was accurate and reproducible, and can be used for the determination of ilexside II in leaves of Ilex cornuta.
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