[Study on diversity of Liriope spicata var. prolifera and its affinis species with ISSR method]

To study the identification of Liriope spicata var. prolifera and its affinis species, which are difficult to be differentiated with routine method, based on ISSR molecular marker technology and explore their relationship.

According to the pre-experiment, to design orthogonal experiment of four levels and five factors to optimize the PCR reaction system. To select randomly 80 primers from ISSR primers sequence table and then screen effective primers from the experiment trough PCR amplifying of all samples. Mark the samples with optimal ISSR conditions, and calculate the similarity coefficient between samples, then build the phylogenetic tree of these samples.

The study established a better ISSR reaction system and the PCR amplification procedure for Liriope spicata var. prolifera and its affinis species, screened out 9 effective primers and achieved ISSR electrophoretic spectrum and phylogenetic tree of 15 samples.

The established ISSR molecular marker techniques can be used for the researches of identification and genetic relationship for some Liriope and Ophiopogon species.