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[Observation of double-stained African green monkey kidney COS-7 cells using total internal reflection double-channel

Xiao-chen Liu1, Li-zhao Guan, Wan-yun Ma

  • 1Key Laboratory for Atomic and Molecular Nanosciences of Ministry of Education, Department of Physics, Tsinghua University, Beijing 100084, China. liuxc06@mails.tsinghua.edu.cn

Guang Pu Xue Yu Guang Pu Fen Xi = Guang Pu
|December 9, 2010
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Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.