Related Experiment Video
Updated: Jun 6, 2026
Evaluation of Biomarkers in Glioma by Immunohistochemistry on Paraffin-Embedded 3D Glioma Neurosphere Cultures
Published on: January 9, 2019
Expression of progestrone receptor and proliferative marker ki 67 in various grades of meningioma
Nasrin Shayanfar1, Masoud Mashayekh, Masoud Mohammadpour
1Department of Pathology, School of Medicine, Iran University of Medical Sciences, Tehran, Iran. nasrinshayanfar@iums.ac.ir
Abstract:
Meningiomas are slow-growing neoplasms which recur locally, their morphologic grading is simple but do not always correlate with patient outcome. The aim of present study is to evaluate the status of progesterone receptor (PR) and proliferation marker Ki67 in various grades of meningioma in a group of Iranian patients. 78 cases of meningioma were selected from the file of a hospital university. All archival H&E stained sections were reviewed and regraded according to WHO criteria. Immunohistochemical analysis for PR and Ki67 was performed on formalin-fixed, paraffin-embedded samples. PR status considered positive if >10% of tumor cell's nuclei were strongly immunoreactive, or if >50% of nuclei were stained with medium intensity. The Ki67 labeling index (LI) is defined as the percentage area with strongest immunostaining. PR were positive in 61/63 (96.8%) of grade I tumors, 2/10 (20%) of grade II, and 0/5 (0%) of grade III tumors. Ki67 LI was %2.98 +/- 2.27 in grade I tumors, %9.30 +/- 5.79 in grade II tumors and %34.00 +/- 5.47 in grade III tumors. For both markers, differences between grade I, II and III tumors were significant (P < 0.001). There was a reverse relationship between mean of Ki67 LI and PR status, with increasing grade of tumor. Evaluation of PR status and Ki67 LI together with conventional histologic evaluation can help in providing more information about the biologic behaviour of meningiomas, especially for those that histological grading is not straightforward.
Insights
Progesterone receptor (PR) and Ki67 proliferation marker levels vary significantly across meningioma grades. PR is high in grade I but low in higher grades, while Ki67 increases with tumor grade, aiding in predicting meningioma behavior.
Area of Science:
- Neuropathology
- Oncology
- Molecular Pathology
Background:
- Meningiomas are primary brain tumors with variable clinical outcomes.
- Morphologic grading of meningiomas does not always correlate with patient prognosis.
- Progesterone receptor (PR) and proliferation markers like Ki67 are potential indicators of tumor behavior.
Purpose of the Study:
- To evaluate the expression of progesterone receptor (PR) and Ki67 in different grades of meningioma.
- To determine the correlation between PR and Ki67 status and meningioma grade in Iranian patients.
- To assess the utility of these markers in predicting meningioma biologic behavior.
Main Methods:
- Retrospective review of 78 meningioma cases.
- Histopathologic re-grading according to WHO criteria.
- Immunohistochemical analysis for PR and Ki67 expression on formalin-fixed, paraffin-embedded samples.
Main Results:
- PR positivity was high in grade I (96.8%) but decreased significantly in grade II (20%) and was absent in grade III (0%).
- Ki67 labeling index (LI) significantly increased with tumor grade: 2.98% in grade I, 9.30% in grade II, and 34.00% in grade III.
- A significant inverse relationship was observed between PR status and Ki67 LI with increasing meningioma grade (P < 0.001).
Conclusions:
- PR status and Ki67 LI are valuable biomarkers that correlate with meningioma grade.
- Combined evaluation of PR, Ki67, and histology improves the understanding of meningioma biologic behavior.
- These markers can aid in prognostication, especially for challenging histological gradings.
