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Studying the Cytoskeleton01:17

Studying the Cytoskeleton

The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...
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Animal and Plant Cell Structure

Animal and plant cells not only differ in their structure, function, and mode of nutrition but also in how they reproduce, specialize, and organize into complex structures.
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Though both plant and animal cells divide by mitosis (for non-gametic cells) and meiosis (for gametic cells), they differ in the specifics of this process. Unlike animal cells, plant cells lack centrosomes — an organelle responsible for organizing the spindle fibers and segregating the chromosomes during cell...
Atomic Force Microscopy01:08

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
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The probe is regarded as the heart of any AFM setup and comprises the...

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Related Experiment Video

Updated: May 25, 2026

Use of Atomic Force Microscopy to Measure Mechanical Properties and Turgor Pressure of Plant Cells and Plant Tissues
11:18

Use of Atomic Force Microscopy to Measure Mechanical Properties and Turgor Pressure of Plant Cells and Plant Tissues

Published on: July 15, 2019

Atomic force microscope observation on ultrastructures in plant cells.

Xin Wang1, Yuliang Zhang, Kaihe Du

  • 1State Key Laboratory of Palaeobiology and Stratigraphy, Nanjing Institute of Geology and Palaeontology, Nanjing 210008, China.

Journal of Nanoscience and Nanotechnology
|December 9, 2010
PubMed
Summary
This summary is machine-generated.

Atomic Force Microscopy (AFM) can now visualize subcellular details in plant cells without sample processing artifacts. This new method offers a faithful and efficient way to apply AFM to biological materials.

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Related Experiment Videos

Last Updated: May 25, 2026

Use of Atomic Force Microscopy to Measure Mechanical Properties and Turgor Pressure of Plant Cells and Plant Tissues
11:18

Use of Atomic Force Microscopy to Measure Mechanical Properties and Turgor Pressure of Plant Cells and Plant Tissues

Published on: July 15, 2019

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05:51

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Published on: March 31, 2022

Characterizing Mechanical Properties of Primary Cell Wall in Living Plant Organs Using Atomic Force Microscopy
09:52

Characterizing Mechanical Properties of Primary Cell Wall in Living Plant Organs Using Atomic Force Microscopy

Published on: May 18, 2022

Area of Science:

  • Biophysics
  • Cell Biology
  • Microscopy

Background:

  • Atomic Force Microscopy (AFM) offers advanced biochemical and biophysical insights beyond traditional SEM and TEM.
  • AFM's application for observing subcellular details within intact cells is limited due to potential artifacts from sample preparation.

Purpose of the Study:

  • To develop and validate a modified AFM method for observing subcellular details in plant cells embedded in resin.
  • To minimize artifacts associated with traditional sample processing for AFM analysis of plant cells.

Main Methods:

  • Observing plant cells directly within their resin blocks using AFM.
  • Comparing AFM results with Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), and Light Microscopy (LM) on identical single cells.

Main Results:

  • The modified AFM method successfully visualized subcellular features in plant cells without significant artifacts.
  • Comparative analysis confirmed the applicability, efficiency, and faithfulness of the new AFM approach.

Conclusions:

  • Observing plant cells embedded in resin using AFM is a viable alternative to traditional methods.
  • This technique enhances the capability of AFM for detailed subcellular analysis in biological samples.