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Related Experiment Video

Updated: Jun 6, 2026

Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes

Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes

Published on: September 26, 2013

Sarcoidosis is a Th1/Th17 multisystem disorder.

Monica Facco¹, Anna Cabrelle, Antonella Teramo

¹Padua University School of Medicine, Department of Clinical and Experimental Medicine, Hematology and Clinical Immunology Section, Via Giustiniani 2, 35128 Padova, Italy.

|December 9, 2010

Summary

T helper 17 (Th17) cells are involved in sarcoidosis pathogenesis, infiltrating lung tissues and contributing to granuloma formation. Their recruitment may be driven by the CCL20 chemokine, suggesting a role in disease progression.

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Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes

Isolation and Th17 Differentiation of Naïve CD4 T Lymphocytes

Published on: September 26, 2013

In Vitro Differentiation of Naive CD4+ T Cells into Pathogenic Th17 Cells in Mouse

In Vitro Differentiation of Naive CD4⁺ T Cells into Pathogenic Th17 Cells in Mouse

Published on: October 25, 2024

Area of Science:

Immunology
Cell Biology
Pulmonology

Background:

Sarcoidosis involves CD4(+) T helper 1 (Th1) lymphocytes and macrophages in affected organs.
Recent evidence suggests Th17 effector CD4(+) T cells may contribute to granuloma formation in sarcoidosis.

Purpose of the Study:

To investigate the role of Th17 cells in the pathogenesis of sarcoidosis.

Main Methods:

Evaluated peripheral and pulmonary Th17 cells using flow cytometry, real-time PCR, immunohistochemistry, and functional assays.
Analyzed sarcoidosis patients across different disease phases and compared them to control subjects.

Main Results:

Th17 cells were found in peripheral blood and bronchoalveolar lavage (BAL) of sarcoidosis patients.
Interleukin-17 (IL-17)-producing CD4(+) T cells infiltrated sarcoid tissues, and IL-17 was expressed by macrophages in granulomas.
Th17 cells showed chemotaxis towards CCL20, the CCR6 ligand, suggesting a mechanism for recruitment.

Conclusions:

Th17 cells play a role in the alveolitic/granuloma and fibrotic phases of sarcoidosis.
CCL20 chemokine may drive the recruitment of Th17 cells in sarcoidosis pathogenesis.