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Updated: Jun 6, 2026

Alternative Methods for the Detection of Superoxide Anion Generation in Platelets
Published on: March 29, 2024
The potential physiological implications of polygalacturonic acid-mediated production of superoxide
Ivan Spasojević1, Jelena Bogdanović Pristov
1Institute for Multidisciplinary Research, University of Belgrade, Belgrade, Serbia. ivan@cms.bg.ac.rs
Abstract:
PGA/OGA/PF represent apoplastic signaling molecules implicated in the control of gene expression and the activity of enzymes involved in defense regulation. However, the underlying mechanisms behind such processes are lacking. Here we unequivocally show using EPR spectroscopy with DEPMPO spin-trap capable of differentiating between •OH and •O(2)(-) that PGA and PF can produce •O(2)(-) by transforming •OH. The potential physiological implications of this unique property are discussed. We propose that PGA/OGA/PF could represent the initiators of redox signaling cascades in stress response, with H(2)O(2) being a downstream secondary messenger.
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