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Highly selective oxidation of benzyl alcohol using engineered Gluconobacter oxydans in biphasic system
Jian Wu1, Ming Hua Li, Jin Ping Lin
1New World Institute of Biotechnology, State Key Lab of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237, China.
Abstract:
The Gluconobacter oxydans M5 with disruption of the pyrroloquinoline quinine-dependent membrane-bound aldehyde dehydrogenase (ALDH) was used for the oxidation of benzyl alcohol. The selectivity toward benzaldehyde showed an obvious increase for the engineered strain, which reached the 67.3%, while the wild strain had only 2.8%. Meantime, the aqueous/isooctane (1:1) biphasic system was used for the further improvement of selectivity. By these methods, nearly 100% selectivity and conversion rate could be obtained within 1 h at the optimum initial benzyl alcohol concentration of 5.0 g/l.
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