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Updated: Jun 6, 2026

Analysis of the c-KIT Ligand Promoter Using Chromatin Immunoprecipitation
Published on: June 27, 2017
Investigation of the interaction between CREB-binding protein and STAT4/STAT6
Mingxiang Zhang1, Zhou Fu, Daiyin Tian
1Department of Respiratory Medicine, Chongqing Medical University, Chongqing 400014, People's Republic of China.
Abstract:
Coactivator CBP (CREB-binding protein) has been implicated in the regulation of transcription for all signal transducer and activator of transcription factors (STATs); however, the mechanism remains unclear. Using yeast two-hybrid screening and immunoprecipitation techniques, we investigated the direct interaction of CBP with STAT4 and STAT6. The full-length CBP and five fragments of CBP (residues 1-436, 529-1200, 1-697, 967-1574 and 1678-2175) were constructed using pGBKT7 vectors, while STAT4, STAT6 and N-terminal deleted STAT4 were constructed using pGADT7 vectors. It was found that STAT4, but not STAT6, interacted directly with the 1678-2175 fragment of CBP containing the ZZ, TAZ2 and SID domain. The N-terminal of STAT4 plays an important role in this interaction since N-terminal deleted STAT4 failed to bind to any CBP fragment. The results were confirmed by immunoprecipitation using HA-tagged STAT4 or STAT6 and c-Myc tagged CBP. This work will contribute to our understanding of the mechanisms of Th cytokine imbalance.
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