Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Fixation and Sectioning01:03

Fixation and Sectioning

Two basic types of preparation are used to visualize specimens with a light microscope: wet mounts and fixed specimens.
The simplest type of preparation is the wet mount, in which the specimen is placed in a drop of liquid on the slide. A liquid specimen can be directly deposited on the slide using a dropper. Solid specimens, such as skin scraping, can be placed on the slide before adding a drop of liquid to prepare the wet mount. Sometimes the liquid is simply water, but stains are often added...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Applicability of the CT Radiomics of Skeletal Muscle and Machine Learning for the Detection of Sarcopenia and Prognostic Assessment of Disease Progression in Patients with Gastric and Esophageal Tumors.

Diagnostics (Basel, Switzerland)·2024
Same author

Patient-specific Predictors of Surgical Delay in a Large Tertiary-care Hospital Operating Room.

Journal of perianesthesia nursing : official journal of the American Society of PeriAnesthesia Nurses·2023
Same author

Concomitant Mental Illnesses Diagnoses and Likelihood of Trauma Recidivism.

Journal of trauma nursing : the official journal of the Society of Trauma Nurses·2023
Same author

Genome wide CRISPR screen for Pasteurella multocida toxin (PMT) binding proteins reveals LDL Receptor Related Protein 1 (LRP1) as crucial cellular receptor.

PLoS pathogens·2022
Same author

Comparison of RT-PCR Cycle Threshold Values from Respiratory Specimens in Symptomatic and Asymptomatic Children with SARS-CoV-2 Infection.

Clinical infectious diseases : an official publication of the Infectious Diseases Society of America·2021
Same author

Comparison of Reverse-Transcription Polymerase Chain Reaction Cycle Threshold Values From Respiratory Specimens in Symptomatic and Asymptomatic Children With Severe Acute Respiratory Syndrome Coronavirus 2 Infection.

Clinical infectious diseases : an official publication of the Infectious Diseases Society of America·2021
Same journal

Evaluation of Diverse Health Professionals' Learning Experience in a Continuing Education Activity for Quality Practices in Molecular Genetic Testing.

Clinical laboratory science : journal of the American Society for Medical Technology·2019
Same journal

Guidelines for Initiating a Research Agenda: Research Design and Dissemination of Results.

Clinical laboratory science : journal of the American Society for Medical Technology·2015
Same journal

Guidelines for Initiating a Research Agenda: Topic Selection and Evidence of Impact.

Clinical laboratory science : journal of the American Society for Medical Technology·2015
Same journal

Autonomy and Privacy in Clinical Laboratory Science Policy and Practice.

Clinical laboratory science : journal of the American Society for Medical Technology·2015
Same journal

The Value of Clinical Laboratory Sciences Research.

Clinical laboratory science : journal of the American Society for Medical Technology·2015
Same journal

From Technical Assistants to Critical Thinkers: From World War II to 2014.

Clinical laboratory science : journal of the American Society for Medical Technology·2015
See all related articles

Related Experiment Video

Updated: Jun 6, 2026

Novel Diagnostics in Revision Arthroplasty: Implant Sonication and Multiplex Polymerase Chain Reaction
10:35

Novel Diagnostics in Revision Arthroplasty: Implant Sonication and Multiplex Polymerase Chain Reaction

Published on: December 3, 2017

Assessing pediatric trauma specimen integrity.

Ruth A Bush1, Teresa Mueller, Beverly Sumwalt

  • 1Research Department (MC 5074), Rady Children's Hospital San Diego, 3020 Children's Way, San Diego, CA 92123, USA. rbush@rchsd.org

Clinical Laboratory Science : Journal of the American Society for Medical Technology
|December 15, 2010
PubMed
Summary
This summary is machine-generated.

Venipuncture is recommended over line draws for collecting blood specimens in trauma patients to minimize sample hemolysis and improve laboratory testing accuracy. This ensures better trauma specimen integrity for critical patient care.

More Related Videos

Intraoperative Assessment of Resection Margins in Oral Cavity Cancer: This is the Way
04:45

Intraoperative Assessment of Resection Margins in Oral Cavity Cancer: This is the Way

Published on: May 10, 2021

Use of Frozen Tissue in the Comet Assay for the Evaluation of DNA Damage
11:30

Use of Frozen Tissue in the Comet Assay for the Evaluation of DNA Damage

Published on: March 24, 2020

Related Experiment Videos

Last Updated: Jun 6, 2026

Novel Diagnostics in Revision Arthroplasty: Implant Sonication and Multiplex Polymerase Chain Reaction
10:35

Novel Diagnostics in Revision Arthroplasty: Implant Sonication and Multiplex Polymerase Chain Reaction

Published on: December 3, 2017

Intraoperative Assessment of Resection Margins in Oral Cavity Cancer: This is the Way
04:45

Intraoperative Assessment of Resection Margins in Oral Cavity Cancer: This is the Way

Published on: May 10, 2021

Use of Frozen Tissue in the Comet Assay for the Evaluation of DNA Damage
11:30

Use of Frozen Tissue in the Comet Assay for the Evaluation of DNA Damage

Published on: March 24, 2020

Area of Science:

  • Clinical Pathology
  • Laboratory Medicine
  • Trauma Care

Background:

  • Accurate laboratory testing is crucial for effective trauma patient management.
  • Specimen quality can be impacted by collection methods, affecting test results.
  • Hemolysis is a common issue in trauma blood samples, potentially leading to inaccurate diagnoses.

Purpose of the Study:

  • To compare the quality of trauma blood specimens collected via line draws versus venipuncture.
  • To identify factors contributing to specimen hemolysis in trauma patients.
  • To provide recommendations for improving blood sample integrity in trauma settings.

Main Methods:

  • Analysis of routinely collected trauma specimens.
  • Comparison of specimen suitability for processing based on draw type (line draw vs. venipuncture).
  • Evaluation of container type (Vacutainer vs. Microtainer) and its association with hemolysis.

Main Results:

  • 13% of all blood draws showed some degree of hemolysis affecting results.
  • Line draws had a significantly higher rate of hemolysis (16%) compared to venipunctures (6%).
  • No statistical association was found between hemolysis and the collector or container type.

Conclusions:

  • Venipuncture is the preferred method for collecting blood specimens for laboratory testing in trauma patients.
  • Implementing venipuncture whenever possible can enhance trauma specimen integrity.
  • Reducing hemolysis is key to ensuring reliable laboratory results for trauma care.