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Updated: Jun 6, 2026
Isolation, Characterization, and Total DNA Extraction to Identify Endophytic Fungi in Mycoheterotrophic Plants
Published on: May 5, 2023
[Isolation, purification and characterization of antifungal protein from rice endophytic bacterim Bacillus subtilis
Xijun Chen1, Juan Li, Qili Sun
1College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China. xjchen@yzu.edu.cn
Objective:
In order to obtain the antagonistic protein of Bacillus subtilis G87 and definitude its characterization.
Methods:
Methods of ammonium sulfate precipitating and column chromatography analyzing were used to isolate and purify the protein.
Results:
A purified protein (peak 6-2-1) was obtained which molecular weight was 50.8 kD by SDS-PAGE and isoelectric point was 5.90 by IEF-PAGE. The antifungal protein contained 0.62% saccharide and some proline or hydroxyproline, but no lipid and aromatic amino acid. The inhibitory activity of the antifungal protein would decreased distinctly at the higher temperature (> or = 60 degrees C) and in the condition of alkalinity (pH > 8), but tolerant to ultraviolet radiation, chloroform, trypsin, proteinase K and pepsin.
Conclusion:
Antifungal protein of Bacillus subtilis G87 was a kind of glycoprotein without aromatic hydrocarbon. It was sensitive to higher temperature and tight alkalinity but not to proteinase analog and ultraviolet radiation et al.
