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Updated: Jun 6, 2026

High-resolution Tandem Mass Spectrometry for Studying Chemical Constituents of Gynura bicolor DC
Published on: February 2, 2024
[Chemical constituents of bear bile]
Qiang Luo1, Quancheng Chen, Yao Wu
1Institute for Biomedical Research, Xiamen University, Xiamen 361005, China.
Objective:
To study the chemical constituents of bear bile.
Method:
The compounds were isolated by repeated column HP20 macroporous adsorption resin, Sephadex LH-20, ODS and silica gel as packing materials. The structures were identified on the basis of extensive spectroscopic data analysis and by comparison of their spectral data reported.
Result:
Nine compounds were identified as 4',7-dihydroxyisoflavone (1), 4',7-dihydroxy-6-methoxyisoflavone (2), 4',6,7-trihydroxyisoflavone (3), 4'-methoxy-7-hydroxyisoflavone (4), tauroursodeoxycholic acid (5), taurochenodeoxycholic acid (6), ursodeoxycholic acid (7), chenodeoxycholic acid (8), cholesterol (9).
Conclusion:
Compounds 1-4 were separated from bear bile for the first time.
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