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Updated: Jun 6, 2026

Electrophoretic Separation of Proteins
Published on: June 12, 2008
Investigation of electrophoretic exclusion method for the concentration and differentiation of proteins
Michelle M Meighan1, Jared Vasquez, Luke Dziubcynski
1Department of Chemistry and Biochemistry, Arizona State University, Tempe, Arizona 85287, United States.
Abstract:
This work presents a technique termed as "electrophoretic exclusion" that is capable of differentiation and concentration of proteins in bulk solution. In this method, a hydrodynamic flow is countered by the electrophoretic velocity to prevent a species from entering into a channel. The separation can be controlled by changing the flow rate or applied electric potential in order to exclude a certain species selectively while allowing others to pass through the capillary. The exclusion of various proteins is investigated using a flow-injection regime of the method. Concentration of myoglobin of up to 1200 times the background concentration in 60 s was demonstrated. Additionally, negatively charged myoglobin was separated from a solution containing negatively charged allophycocyanin. Cationic cytochrome c was also differentiated from a solution with allophycocyanin. The ability to differentially transport species in bulk solution enables parallel and serial separation modes not available with other separations schemes.
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