Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Neural network fusion: a novel CT-MR Aortic Aneurysm image segmentation method.

Proceedings of SPIE--the International Society for Optical Engineering·2018

Same author

Involved Small Arteries in Patients Who Underwent Microvascular Decompression for Hemifacial Spasm.

World neurosurgery·2018

Same author

Revealing the importance of non-thermal effect to strengthen hydrolysis of cellulose by synchronous cooling assisted microwave driving.

Carbohydrate polymers·2018

Same author

A Dedicated 36-Channel Receive Array for Fetal MRI at 3T.

IEEE transactions on medical imaging·2018

Same author

Rh(III)-Catalyzed Enaminone-Directed C-H Coupling with α-Diazo-α-phosphonoacetate for Reactivity Discovery: Fluoride-Mediated Dephosphonation for C-C Coupling Reactions.

Organic letters·2018

Same author

Rh(III)-Catalyzed Enaminone-Directed Alkenyl C-H Activation for the Synthesis of Salicylaldehydes.

Organic letters·2018

Search research articles

Related Experiment Video

Updated: Jun 6, 2026

An Aptamer-based Sensor for Unchelated Gadolinium(III)

An Aptamer-based Sensor for Unchelated Gadolinium(III)

Published on: January 9, 2017

Ultra-sensitive fluorescent sensor for Hg2+ based on a donor-acceptor-donor framework.

Xingqiang Liu¹, Xin Shu, Xin Zhou

¹Department of Polymer Science and Engineering, School of Chemistry and Chemical Engineering, State Key Laboratory of Coordination Chemistry, Nanjing National Laboratory of Microstructures, Nanjing University, Nanjing 210093, China.

The Journal of Physical Chemistry. A

|December 15, 2010

Summary

A novel fluoroionophore probe (E1) offers ultrasensitive detection of mercury ions (Hg2+) in water/THF, achieving detection limits as low as 4.4 × 10(-14) M. This donor-acceptor-donor probe shows a significant fluorescence quenching response to Hg2+.

More Related Videos

Real-Time Monitoring of Aurora kinase A Activation using Conformational FRET Biosensors in Live Cells

Real-Time Monitoring of Aurora kinase A Activation using Conformational FRET Biosensors in Live Cells

Published on: July 30, 2020

Real-time Monitoring of Ligand-receptor Interactions with Fluorescence Resonance Energy Transfer

Real-time Monitoring of Ligand-receptor Interactions with Fluorescence Resonance Energy Transfer

Published on: August 20, 2012

Related Experiment Videos

Last Updated: Jun 6, 2026

An Aptamer-based Sensor for Unchelated Gadolinium(III)

An Aptamer-based Sensor for Unchelated Gadolinium(III)

Published on: January 9, 2017

Real-Time Monitoring of Aurora kinase A Activation using Conformational FRET Biosensors in Live Cells

Real-Time Monitoring of Aurora kinase A Activation using Conformational FRET Biosensors in Live Cells

Published on: July 30, 2020

Real-time Monitoring of Ligand-receptor Interactions with Fluorescence Resonance Energy Transfer

Real-time Monitoring of Ligand-receptor Interactions with Fluorescence Resonance Energy Transfer

Published on: August 20, 2012

Area of Science:

Supramolecular Chemistry
Analytical Chemistry
Materials Science

Background:

Development of selective and sensitive fluorescent probes is crucial for environmental monitoring and biological imaging.
Mercury ions (Hg2+) are highly toxic environmental pollutants requiring accurate detection methods.
Fluoroionophores with donor-acceptor frameworks offer potential for sensitive ion detection.

Purpose of the Study:

To synthesize and characterize a new fluoroionophore, E-4,4'-di(N-(2-pyridyl)amino)stilbene (E1), with a donor-acceptor-donor framework.
To investigate the fluorescence response of E1 towards Hg2+ in an aqueous-organic solvent mixture.
To establish the detection limit and explore the sensing mechanism of E1 for Hg2+.

Main Methods:

Synthesis of the fluoroionophore E1 and a control molecule E4.
Spectroscopic characterization using UV-vis absorption, fluorescence spectroscopy, and nuclear magnetic resonance (NMR) spectroscopy.
Density functional theory (DFT) calculations to elucidate the sensing mechanism.

Main Results:

The fluoroionophore E1 exhibits an ultrasensitive fluorescence quenching response to Hg2+ in H2O/THF.
Coordination of Hg2+ to E1 forms a 2:1 complex, enabling Hg2+ detection at concentrations as low as 4.4 × 10(-14) M.
A control molecule (E4) with a donor-acceptor structure showed a significantly weaker quenching response to Hg2+.

Conclusions:

The donor-acceptor-donor fluoroionophore E1 is highly effective for the ultrasensitive detection of Hg2+.
The observed fluorescence quenching is attributed to the coordination of Hg2+ to the pyridylamino groups.
E1 represents a promising fluorescent probe for the trace detection of mercury ions.