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Related Experiment Video

Updated: Jun 6, 2026

Low-cost Custom Fabrication and Mode-locked Operation of an All-normal-dispersion Femtosecond Fiber Laser for Multiphoton Microscopy

Low-cost Custom Fabrication and Mode-locked Operation of an All-normal-dispersion Femtosecond Fiber Laser for Multiphoton Microscopy

Published on: November 22, 2019

Laser-controlled fluorescence in two-level systems.

Jamie M Leeder¹, David S Bradshaw, David L Andrews

¹School of Chemistry, University of East Anglia, Norwich NR4 7TJ, United Kingdom.

The Journal of Physical Chemistry. B

|December 15, 2010

Summary

This summary is machine-generated.

This study demonstrates a new all-optical method to control fluorescence emission using lasers. This technique offers novel ways to gain chemically specific information from fluorescent markers like quantum dots.

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Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation

Internalization and Observation of Fluorescent Biomolecules in Living Microorganisms via Electroporation

Published on: February 8, 2015

Area of Science:

Optics and Photonics
Biophysics
Materials Science

Background:

Fluorescence is a key tool in chemical analysis and imaging.
Controlling fluorescence properties is crucial for advanced applications.
Existing methods for fluorescence modification are limited.

Purpose of the Study:

To analyze an all-optical process for controlling fluorescence emission.
To explore the potential of laser-controlled nonlinear optical processes.
To provide a theoretical framework for novel fluorescence imaging techniques.

Main Methods:

Utilizing a two-level emission model for theoretical analysis.
Applying off-resonant probe laser pulses during excited-state decay.
Developing expressions for laser-controlled fluorescence anisotropy.

Main Results:

Demonstrated controllable modification of fluorescence decay rates.
Showcased the ability to generate chemically specific information.
Presented expressions for fluorescence anisotropy in randomly oriented samples.
Showed potential for inducing fluorescence from normally non-radiative decay pathways.

Conclusions:

The all-optical control of fluorescence is theoretically feasible and practically relevant.
This method enhances the utility of fluorescent markers in analysis and imaging.
Potential applications span from quantum dots to green fluorescence protein imaging.