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Related Experiment Video

Updated: Jun 6, 2026

Microfluidic Chip Fabrication and Method to Detect Influenza
09:43

Microfluidic Chip Fabrication and Method to Detect Influenza

Published on: March 26, 2013

Predicting viruses accurately by a multiplex microfluidic loop-mediated isothermal amplification chip.

Xueen Fang, Hui Chen, Shaoning Yu

    Analytical Chemistry
    |December 15, 2010
    PubMed
    Summary

    A novel octopus-like multiplex microfluidic loop-mediated isothermal amplification (mμLAMP) assay enables rapid, point-of-care analysis of multiple genes. This cost-effective method accurately detects viruses and differentiates influenza A substrains.

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    Area of Science:

    • Molecular Biology
    • Biotechnology
    • Diagnostics

    Background:

    • Multiplex gene assays are crucial in research and clinical diagnostics.
    • Existing methods like multiplex PCR and DNA microarrays are not ideal for point-of-care or resource-limited settings.
    • There is a need for rapid, simple, and cost-effective multiplex gene analysis tools for field applications.

    Purpose of the Study:

    • To develop a novel octopus-like multiplex microfluidic loop-mediated isothermal amplification (mμLAMP) assay.
    • To enable rapid, qualitative, and quantitative multiplex gene analysis at the point-of-care.
    • To provide a robust method for virus prediction and identification.

    Main Methods:

    • Development of an octopus-like microfluidic chip for multiplex isothermal amplification.

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    Related Experiment Videos

    Last Updated: Jun 6, 2026

    Microfluidic Chip Fabrication and Method to Detect Influenza
    09:43

    Microfluidic Chip Fabrication and Method to Detect Influenza

    Published on: March 26, 2013

    Visual Detection of Multiple Nucleic Acids in a Capillary Array
    08:56

    Visual Detection of Multiple Nucleic Acids in a Capillary Array

    Published on: November 15, 2017

    Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1
    06:18

    Multiplexed Isothermal Amplification Based Diagnostic Platform to Detect Zika, Chikungunya, and Dengue 1

    Published on: March 13, 2018

  • Utilizing loop-mediated isothermal amplification (LAMP) for high sensitivity and specificity.
  • Testing the assay's performance for differentiating influenza A substrains and identifying swine viruses.

    • Main Results:

    • The mμLAMP assay demonstrated high specificity, operational simplicity, and cost/time-effectiveness.
    • Achieved a detection limit of less than 10 copies/μL in 2 μL samples within 0.5 hours.
    • Successfully differentiated three human influenza A substrains and identified eight swine viruses.

    Conclusions:

    • The developed mμLAMP assay is a valuable tool for point-of-care multiplex gene diagnostics.
    • This technology offers a robust and efficient approach for rapid virus detection and subtyping.
    • The assay's performance makes it suitable for resource-limited settings and clinical diagnostics.