Related Experiment Video
Updated: Jun 6, 2026

Heuristic Mining of Hierarchical Genotypes and Accessory Genome Loci in Bacterial Populations
Published on: December 7, 2021
A combined global and local approach to elucidate spatial organization of the Mycobacterial ParB-parS partition
Barnali N Chaudhuri1, Sayan Gupta, Volker S Urban
1Hauptman Woodward Institute, Buffalo, NY 14203, USA. bchaudhuri@hwi.buffalo.edu
Abstract:
Combining diverse sets of data at global (size, shape) and local (residue) scales is an emerging trend for elucidating the organization and function of the cellular assemblies. We used such a strategy, combining data from X-ray and neutron scattering with H/D-contrast variation and X-ray footprinting with mass spectrometry, to elucidate the spatial organization of the ParB-parS assembly from Mycobacterium tuberculosis. The ParB-parS participates in plasmid and chromosome segregation and condensation in predivisional bacterial cells. ParB polymerizes around the parS centromere(s) to form a higher-order assembly that serves to recruit cyto-skeletal ParA ATPases and SMC proteins for chromosome segregation. A hybrid model of the ParB-parS was built by combining and correlating computational models with experiment-derived information about size, shape, position of the symmetry axis within the shape, internal topology, DNA-protein interface, exposed surface patches, and prior knowledge. This first view of the ParB-parS leads us to propose how ParB spread on the chromosome to form a larger assembly.
More Related Videos
14:58Identification of Protein Complexes in Escherichia coli using Sequential Peptide Affinity Purification in Combination with Tandem Mass Spectrometry
Published on: November 12, 2012
07:42A Novel Microdissection Approach to Recovering Mycobacterium tuberculosis Specific Transcripts from Formalin Fixed Paraffin Embedded Lung Granulomas
Published on: June 5, 2014
Related Concept Videos
Modern Molecular Taxonomy
Cytoskeletal Proteins in Bacteria
Assembly of Complex Microtubule Structures
Eukaryotic Compartmentalization
For example, lysosomes in the animal cells...
Eukaryotic Compartmentalization
For example, lysosomes in the animal cells...
Eukaryotic Compartmentalizations
For example, lysosomes in the animal cells...