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An In Vivo Assessment of Blood-Brain Barrier Disruption in a Rat Model of Ischemic Stroke
Published on: March 11, 2018
Brain extracellular fluid protein changes in acute stroke patients
Loï Dayon1, Natacha Turck, Teresa Garcí-Berrocoso
1Biomedical Proteomics Group, Department of Structural Biology and Bioinformatics, Faculty of Medicine, University of Geneva, Geneva, Switzerland.
Journal of Proteome Research
|December 15, 2010
Summary
Investigating brain extracellular fluids in stroke patients revealed elevated protein levels in damaged areas. These findings suggest extracellular fluid is a promising source for discovering new blood stroke biomarkers.
Area of Science:
- Neuroscience
- Biochemistry
- Proteomics
Background:
- Ischemic stroke causes significant brain damage.
- Identifying reliable biomarkers for stroke diagnosis and prognosis is crucial.
- Brain extracellular fluid (ECF) contains proteins reflecting local tissue status.
Purpose of the Study:
- To investigate changes in protein levels in human brain ECF of acute stroke patients.
- To identify potential protein biomarkers for ischemic brain damage.
Main Methods:
- Microdialysis was used to collect ECF from infarct core, penumbra, and contralateral regions in stroke patients.
- Shotgun proteomic analysis with isobaric tagging and mass spectrometry was employed for protein quantification.
- ELISA was used to validate specific protein levels in blood samples.
Main Results:
- 53 proteins showed increased levels in infarct core or penumbra compared to contralateral regions.
- Glutathione S-transferase P (GSTP1), peroxiredoxin-1 (PRDX1), and protein S100-B (S100B) were significantly elevated in stroke patient blood.
- GSTP1, PRDX1, and S100B showed 8-fold, 20-fold, and 11-fold increases, respectively, in stroke patients' blood.
Conclusions:
- ECF is a valuable source for identifying proteins indicative of ischemic brain injury.
- Elevated levels of GSTP1, PRDX1, and S100B in blood suggest their potential as stroke biomarkers.
- Further research into ECF proteins could lead to improved diagnostic tools for stroke.
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