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Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA
Published on: September 10, 2013
Nucleosome positioning pattern derived from oligonucleotide compositions of genomic sequences
Alexandra E Rapoport1, Z M Frenkel, E N Trifonov
1Genome Diversity Center, Institute of Evolution, University of Haifa, Mount Carmel, Haifa 31905, Israel. arapcom@gmail.com
Journal of Biomolecular Structure & Dynamics
|December 15, 2010
Summary
Nucleosome positioning patterns are vital for chromatin studies. This research confirms a universal DNA bendability pattern (CRAAAATTTTYG) across 13 eukaryotic genomes, validating previous findings.
Area of Science:
- Genomics
- Molecular Biology
- Biophysics
Background:
- Nucleosome positioning is essential for understanding chromatin structure and function.
- Previous studies derived matrix and linear forms of nucleosome positioning patterns, identifying a specific DNA motif (CGRAAATTTYCG).
Purpose of the Study:
- To reconstruct DNA bendability patterns for 13 eukaryotic genomes.
- To validate the universality of the established nucleosome DNA bendability motif.
Main Methods:
- A novel approach involving the extension of highest frequency trinucleotides was employed.
- Analysis of trinucleotide frequencies across 13 eukaryotic genomes.
Main Results:
- A consensus bendability pattern, CRAAAATTTTYG, was reconstructed.
- The reconstructed pattern strongly conforms to the previously identified CGRAAATTTYCG motif.
- This finding supports the universality of the nucleosome DNA bendability pattern.
Conclusions:
- The study confirms the existence of a universal DNA bendability pattern crucial for nucleosome positioning.
- The novel reconstruction method validates the identified motif across diverse eukaryotic genomes.
- This research enhances our understanding of DNA-protein interactions in chromatin organization.
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02:33The Nucleosome
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DNA is wound twice around a protein complex called histone core, that consist of 8 histone proteins. This complex...
02:33The Nucleosome
DNA in a human cell is almost 2m long and it is packed inside a tiny nucleus that is only a few microns in diameter. The level of compaction of DNA inside the nucleus is astonishing. It is organized into several sequentially higher levels of compaction to fit into such a tiny space. The most compact form of DNA is a chromosome that can be seen under a microscope in a dividing cell.
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Nucleosomes are the DNA-histone complex, where the DNA strand is wound around the histone core. The histone core is an octamer containing two copies of H2A, H2B, H3, and H4 histone proteins.
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