Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

ER Retrieval Pathway01:45

ER Retrieval Pathway

In the secretory pathway, vesicles transport proteins from one cellular compartment to another in forward transport to deliver the protein to its correct location. Occasionally, misfolded proteins and incorrect proteins escape their original compartments, and a retrieval pathway is used to return the escaped proteins to their original compartment.
The ER uses many checkpoints to prevent the entry of incorrectly folded or a resident protein as cargo onto a transport vesicle. These mechanisms...
Protein Translocation Machinery on the ER Membrane01:28

Protein Translocation Machinery on the ER Membrane

The translocon complex situated on the ER membrane is the main gateway for the protein secretory pathway. It facilitates the transport of nascent peptides into the ER lumen and their insertion into the ER membrane.
Sec61 protein conducting channel
In eukaryotes, the translocon complex comprises a core heterotrimeric translocator channel called the Sec61 complex. This channel includes three transmembrane proteins, Sec61α, Sec61β, and Sec61γ, and is the largest subunit of the translocon complex.
Load along a Single Axis01:29

Load along a Single Axis

In structural engineering, the analysis of beams subjected to varying loads is a critical aspect of understanding the behavior and performance of these structural elements. A common scenario involves a beam subjected to a combination of different load distributions.
Consider a beam of length L subjected to a varying load, which is a combination of parabolic and trapezoidal load distribution along the x-axis. In this case, it is essential to determine the resultant loads, their locations, and...
Assembly of the Lipid Bilayer in the ER01:28

Assembly of the Lipid Bilayer in the ER

Biological membranes are more than just a barrier separating cell cytoplasm from the outside environment. They are highly dynamic and help maintain the integrity and physiological stability of the cells as well as membrane-bound organelles. Membranes also play vital roles in cell-to-cell and intracellular communication.
A large chunk of any biological membrane is composed of phospholipids. These lipids have a heterogeneous distribution across different subcellular organelles and even between...
Eccentric Loading01:16

Eccentric Loading

Eccentric loading is a crucial concept in the study of structural engineering and mechanics, particularly when analyzing the stability and stress distribution in columns. Unlike centric loading, where the force is applied along the centroidal axis, causing uniform compression, eccentric loading occurs when a force is applied off-center. This off-center application introduces not only direct compressive stress but also bending stress, significantly influencing the column's behavior under load.
Impact Loading01:19

Impact Loading

Impact loading occurs when a moving object collides with a stationary structure, such as a rod with a uniform cross-sectional area fixed at one end. Under these conditions, the rod absorbs the kinetic energy from the striking object, leading to deformation and subsequent stress development. As the rod returns to its original position and reaches maximum stress, the absorbed energy, initially manifested as kinetic energy, transforms entirely into strain energy.
In cases of elastic deformation,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Water soluble fuel compounds impair metabolism in benthic foraminifera: Ecotoxicological observations at cellular level.

The Science of the total environment·2026
Same author

Minimally Destructive Radiocarbon Dating of Bone.

Rapid communications in mass spectrometry : RCM·2026
Same author

The short isoform of Tango1 is dispensable for zebrafish survival but is required for skeletal patterning and integrity.

Biology open·2025
Same author

Autophagy regulates endosymbiont distribution in early <i>Drosophila</i> embryogenesis.

Autophagy reports·2025
Same author

Biosorption of heavy metals by microalgae: Hazardous side effects for marine organisms.

Chemosphere·2025
Same author

Bacterial extracellular vesicles as intranasal postbiotics: Detailed characterization and interaction with airway cells.

Journal of extracellular vesicles·2024
Same journal

Correction.

Molecular membrane biology·2020
Same journal

Potassium channels and their role in glioma: A mini review.

Molecular membrane biology·2020
Same journal

Spatial organization of palmitoyl acyl transferases governs substrate localization and function.

Molecular membrane biology·2020
Same journal

Uncoupling protein 2 prevents ischaemia reperfusion injury through the regulation ROS/NF-κB signalling in mice.

Molecular membrane biology·2019
Same journal

Zinc uptake in the Basidiomycota: Characterization of zinc transporters in <i>Ustilago maydis</i>.

Molecular membrane biology·2019
Same journal

The KdpFABC complex - K<sup>+</sup> transport against all odds.

Molecular membrane biology·2019
See all related articles

Related Experiment Video

Updated: Jun 6, 2026

Cargo Loading onto Kinesin Powered Molecular Shuttles
09:00

Cargo Loading onto Kinesin Powered Molecular Shuttles

Published on: November 3, 2010

Cargo loading at the ER.

Katy Schmidt1, David J Stephens

  • 1Cell Biology Laboratories, School of Biochemistry, Medical Sciences Building, University of Bristol, Bristol, UK. k.schmidt@bristol.ac.uk

Molecular Membrane Biology
|December 15, 2010
PubMed
Summary
This summary is machine-generated.

Newly synthesized proteins are trafficked via the early secretory pathway, crucial for cell and tissue organization. Understanding ER export mechanisms, especially for large molecules, is vital for human development and disease research.

More Related Videos

Loading Drosophila Nerve Terminals with Calcium Indicators
15:16

Loading Drosophila Nerve Terminals with Calcium Indicators

Published on: July 30, 2007

Single-Molecule Imaging of Nuclear Transport
12:13

Single-Molecule Imaging of Nuclear Transport

Published on: June 9, 2010

Related Experiment Videos

Last Updated: Jun 6, 2026

Cargo Loading onto Kinesin Powered Molecular Shuttles
09:00

Cargo Loading onto Kinesin Powered Molecular Shuttles

Published on: November 3, 2010

Loading Drosophila Nerve Terminals with Calcium Indicators
15:16

Loading Drosophila Nerve Terminals with Calcium Indicators

Published on: July 30, 2007

Single-Molecule Imaging of Nuclear Transport
12:13

Single-Molecule Imaging of Nuclear Transport

Published on: June 9, 2010

Area of Science:

  • Cell Biology
  • Molecular Biology
  • Biochemistry

Background:

  • The early secretory pathway is essential for intracellular and tissue organization.
  • Mutations in ER export machinery components are linked to various human diseases.
  • Current research often focuses on small, diffusible cargoes, limiting understanding of larger complex export.

Purpose of the Study:

  • To discuss molecular mechanisms of cargo selection during ER vesicle budding.
  • To highlight challenges in exporting large macromolecular complexes like collagens and lipoproteins.
  • To emphasize the necessity of metazoan models for understanding ER export in development and disease.

Main Methods:

  • Review of in vitro reconstitution assays.
  • Analysis of data from unicellular organisms (budding yeast).
  • Examination of mammalian cell culture studies.
  • Integration of findings from metazoan models.

Main Results:

  • Current in vitro and cell culture methods are limited to small cargo analysis.
  • Export of large macromolecular complexes from the ER poses selectivity and efficiency challenges.
  • Metazoan models are crucial for translating ER export knowledge to human development and disease.

Conclusions:

  • Understanding ER export mechanisms is critical for addressing human diseases.
  • The export of large macromolecular complexes requires specific mechanisms not fully elucidated.
  • Further research using metazoan models will illuminate the role of ER export in development and disease progression.