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Related Concept Videos

Proteomics01:33

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term proteomics...
SDS-PAGE01:27

SDS-PAGE

Gel electrophoresis is a method that separates biological macromolecules like nucleic acids or proteins by forcing them to pass through a gel matrix under an electric field.
A variation of gel electrophoresis, termed  polyacrylamide gel electrophoresis (PAGE), is commonly used for separating proteins according to their molecular size by passing them through a polyacrylamide gel. Because of the varying charges associated with amino acid side chains, PAGE can be used to separate intact proteins...

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Related Experiment Video

Updated: Jun 6, 2026

Proteomic Profiling of Macrophages by 2D Electrophoresis
07:53

Proteomic Profiling of Macrophages by 2D Electrophoresis

Published on: November 4, 2014

2D electrophoresis-based expression proteomics: a microbiologist's perspective.

Isabel Sá-Correia1, Miguel C Teixeira

  • 1Institute for Biotechnology and Bioengineering, Biological Sciences Research Group, Centro de Engenharia Biológica e Química, Instituto Superior Técnico, Technical University of Lisbon, Av. Rovisco Pais, Lisbon, Portugal. isacorreia@ist.utl.pt

Expert Review of Proteomics
|December 15, 2010
PubMed
Summary
This summary is machine-generated.

Quantitative proteomics using 2D electrophoresis (2-DE) advances molecular microbial physiology. This technique offers insights into post-translational modifications and toxicoproteomics, aiding systems microbiology research.

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Last Updated: Jun 6, 2026

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An Aquatic Microbial Metaproteomics Workflow: From Cells to Tryptic Peptides Suitable for Tandem Mass Spectrometry-based Analysis

Published on: September 15, 2015

Area of Science:

  • Microbiology
  • Proteomics
  • Systems Biology

Background:

  • Quantitative proteomics using 2D electrophoresis (2-DE) and peptide mass fingerprinting is a key method in microbiology.
  • Advances in 2-DE have improved protein resolution, sensitivity, and accuracy for global expression analysis.

Purpose of the Study:

  • To discuss the exploitation of 2-DE quantitative proteomics in molecular microbial physiology and systems microbiology.
  • To highlight the potential of 2-DE for studying post-translational modifications and toxicoproteomics.
  • To provide guidelines for data exploitation and integration in expression proteomics.

Main Methods:

  • 2D electrophoresis (2-DE) coupled with peptide mass fingerprinting for quantitative proteomic analysis.
  • Exploitation of expression proteomics data using computational tools.
  • Integration of proteomics data with other genome-wide expression information.

Main Results:

  • 2-DE based quantitative proteomics continues to be a fundamental tool for microbiologists.
  • The technique offers insights into post-translational modifications at a proteome-wide level.
  • Toxicoproteomics emerges as a powerful tool for monitoring toxicological responses.

Conclusions:

  • 2-DE based quantitative proteomics is crucial for advancing molecular systems microbiology.
  • The approach provides a comprehensive view of proteome changes in response to environmental or genetic factors.
  • Case studies demonstrate the application of expression proteomics for mechanistic insights into toxicant responses.