[Quantification of human serum apolipoprotein A II by radial immunodiffusion]

A radial immunodiffusion assay (RID) for human serum apolipoprotein A II (apo A II) was developed. Antisera to human apoA II were raised in goats with purified apoA II. The antibody-gel plate was prepared with 1% agarose. 5 microliters of each sample was added in a well. Diffusion was performed at 25 degrees C for 48 h. The minimum measurable concentration of apoA II was 58ng. The standard curve with a working range of 17.3-69 mg/dl was plotted. The coefficients of variation of within and between plates were 1.5-4.6% and 5.6% respectively. The recovery of the assay was 90.3-102.0%. The concentrations of apoA II for healthy men and women in Chengdu area were 26.7 +/- 4.4 mg/dl (n = 45) and 26.6 +/- 4.6 mg/dl (n = 31) respectively.