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Related Concept Videos

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview01:19

Inductively Coupled Plasma–Mass Spectrometry (ICP–MS): Overview

In inductively coupled plasma–mass spectrometry (ICP–MS), an inductively coupled plasma (ICP) torch is used as an atomizer and ionizer. Solid samples are dissolved and volatilized before being introduced into the high-temperature argon plasma, while solution samples are nebulized and passed through the high-temperature argon plasma. Plasma dissociates the analytes and ionizes their component atoms to form a mixture of positive ions and molecular species. The positive ions are then passed on to...
Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences01:20

Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences

Inductively coupled plasma–mass spectrometry (ICP–MS) is a highly selective and sensitive technique for accurate elemental analysis. Though the analysis of ICP–MS mass spectra is comparatively straightforward, it is affected by spectroscopic and non-spectroscopic interferences. Spectroscopic interferences arise when the plasma contains ionic species with an m/z value the same as the analyte ion. Spectroscopic interference can be categorized as isobaric, polyatomic ions, and refractory oxide ion...
Inductively Coupled Plasma Atomic Emission Spectroscopy: Principle01:19

Inductively Coupled Plasma Atomic Emission Spectroscopy: Principle

Inductively coupled plasma (ICP) is the most widely used plasma source in atomic emission spectroscopy (AES), also known as Inductively Coupled Plasma Optical Emission Spectroscopy (ICP-OES). The ICP source, or torch, consists of three concentric quartz tubes with argon gas flowing through them. A spark from a Tesla coil initiates the ionization of argon, generating a high-temperature plasma.
The ions and electrons produced interact with the fluctuating magnetic field created by a water-cooled...
Tandem Mass Spectrometry01:21

Tandem Mass Spectrometry

Tandem mass spectrometry is a technique that uses multiple mass analyzers in series to obtain a higher selectivity and reduce chemical noise during analyte detection. Instruments with multiple analyzers separated by an interaction cell enable secondary fragmentation and selected study of the fragment ions.Secondary fragmentations occur in the interaction cell and can be induced by various factors. Fragmentation induced by collision with inert gases, such as N2, Ar, He, etc., is called...
High-Performance Liquid Chromatography: Types of Detectors01:15

High-Performance Liquid Chromatography: Types of Detectors

The role of the detectors in High-Performance Liquid Chromatography (HPLC) is to analyze the solutes as they exit from the chromatographic column. The detector recognizes the solute's property and generates corresponding electrical signals, which are converted into a readable graph of the detector's response versus elution time called a chromatogram at the computer. There are several types of HPLC detectors, each with its own advantages and limitations, depending on the analyte properties and...
Mass Spectrometry: Complex Analysis01:21

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...

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Automated Sample Multiplexing by using Combined Precursor Isotopic Labeling and Isobaric Tagging (cPILOT)
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Published on: December 18, 2020

ICPD-a new peak detection algorithm for LC/MS.

Jianqiu Zhang1, William Haskins

  • 1Department of Electrical Engineering, University of Texas at San Antonio, Texas, USA. michelle.zhang@utsa.edu

BMC Genomics
|December 15, 2010
PubMed
Summary
This summary is machine-generated.

A new Information Combining Peak Detection (ICPD) method improves protein biomarker discovery by enhancing the identification of low abundance proteins in Liquid Chromatography/Mass Spectrometry (LC/MS) data, overcoming a key challenge in proteomics.

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Area of Science:

  • Proteomics
  • Biomarker Discovery
  • Analytical Chemistry

Background:

  • Label-free Liquid Chromatography/Mass Spectrometry (LC/MS) is vital for protein identification and quantification in research.
  • Low abundance proteins are increasingly recognized as critical biomarkers.
  • Computational identification of low abundance proteins is hindered by noise and variability in LC/MS data.

Purpose of the Study:

  • To develop a novel computational method for improved protein identification in LC/MS.
  • To address the challenge of detecting low abundance proteins in complex proteomic samples.
  • To enhance the accuracy of protein biomarker discovery.

Main Methods:

  • Proposed a new peak detection algorithm named Information Combining Peak Detection (ICPD).
  • ICPD leverages peptide isotope patterns observed across multiple MS scans.
  • Combines information from multiple scans to estimate peptide existence likelihood and utilizes an isotope pattern matching score for peak detection.

Main Results:

  • The ICPD method demonstrates improved peak detection accuracy for low abundance proteins.
  • Evaluated performance using protein standards comprising 48 known proteins.
  • Achieved better accuracy compared to existing LC/MS peak detection techniques.

Conclusions:

  • ICPD offers enhanced accuracy for identifying low abundance proteins in LC/MS.
  • The method shows promise in overcoming bottlenecks in protein biomarker discovery.
  • ICPD represents a significant advancement in computational proteomics.