Related Experiment Video
Updated: Jun 6, 2026

Rejection of Fluorescence Background in Resonance and Spontaneous Raman Microspectroscopy
Published on: May 18, 2011
Revisiting with updated hardware an old spectroscopic technique: circularly polarized luminescence
Ettore Castiglioni1, Sergio Abbate, Giovanna Longhi
1Dipartimento di Scienze Biomediche e Biotecnologie, Università di Brescia, Viale Europa 11, 25123 Brescia, Italy. ettore.castiglioni@jasco-europe.com
Abstract:
Among the various chiroptical spectroscopic techniques available today, circularly polarized luminescence (CPL) plays a minor role and is still used by a limited number of specialists. The cost of the few commercial instruments available and the complexity of homemade apparatuses have strongly limited the widespread application of this technique. New technological approaches, such as the use of light-emitting diode (LED) sources, may significantly simplify the required instrumentation and encourage new potential users. Calibration procedure and the quantitative determination of CPL and total luminescence intensity as well as the corresponding g ratio are described.
Related Concept Videos
Photoluminescence: Applications
Fluorescence and Phosphorescence: Instrumentation
Photoluminescence: Fluorescence and Phosphorescence
A pair of electrons in a...
Raman Spectroscopy Instrumentation: Overview
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...
IR Spectroscopy: Molecular Vibration Overview
Stretching vibrations are vibrational motions that occur along the bond line, changing the bond length or distance between two bonded atoms. They are further distinguished as symmetric or asymmetric. In symmetric stretching, the...
Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview

