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Composite Scaffolds of Interfacial Polyelectrolyte Fibers for Temporally Controlled Release of Biomolecules
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Published on: August 19, 2015

A poloxamine-polylysine acrylate scaffold for modular tissue engineering.

Ema C Ciucurel1, Michael V Sefton

  • 1a Department of Chemical Engineering and Applied Chemistry, Institute of Biomaterials and Biomedical Engineering, University of Toronto, Toronto, ON, Canada.

Journal of Biomaterials Science. Polymer Edition
|December 15, 2010
PubMed
Summary

A novel poloxamine-polylysine acrylate (PPA) polymer was synthesized to improve tissue engineering scaffolds. This new biomaterial enhances endothelial cell adhesion, offering promising applications in regenerative medicine.

Keywords:
HYDROGELSMODULAR TISSUE ENGINEERINGPHOTO-CROSS-LINKABLE SCAFFOLDSPOLOXAMINEPOLYLYSINE

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Published on: October 3, 2014

Area of Science:

  • Biomaterials Science
  • Polymer Chemistry
  • Tissue Engineering

Background:

  • Poloxamine-based polymers are utilized in tissue engineering.
  • Enhancing endothelial cell attachment is crucial for scaffold biocompatibility.
  • Photo-cross-linkable materials offer precise control over scaffold architecture.

Purpose of the Study:

  • To synthesize a novel polymer, poloxamine-polylysine acrylate (PPA), for tissue engineering applications.
  • To improve endothelial cell attachment on poloxamine-based hydrogel scaffolds.
  • To develop a photo-cross-linkable biomaterial with tunable properties.

Main Methods:

  • Synthesis of PPA involved three reaction steps: acrylation of polylysine, activation of poloxamine hydroxyl groups via tresylation, and subsequent reaction between the two components.
  • Characterization of intermediates and the final PPA product was performed using techniques including 1H-NMR, ICP-AES, and CHN elemental analysis.
  • Photo-cross-linking of PPA with poloxamine methacrylate was achieved using 365 nm UV light and a photoinitiator to form hydrogels.

Main Results:

  • The synthesized PPA polymer successfully conferred endothelial cell attachment properties to the poloxamine-based polymer.
  • Photo-cross-linking of PPA and poloxamine methacrylate resulted in stable hydrogel scaffolds.
  • The PPA-modified hydrogels demonstrated enhanced endothelial cell adhesion compared to unmodified scaffolds.

Conclusions:

  • Poloxamine-polylysine acrylate (PPA) is a viable biomaterial for enhancing cell adhesion in tissue engineering scaffolds.
  • The synthesis strategy allows for the incorporation of other functional peptides to tailor scaffold properties.
  • This approach holds potential for developing advanced biomaterials for regenerative medicine applications.