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Up-regulated expression of Notch1 and Jagged1 in human colon adenocarcinoma

J Gao1, J Liu, D Fan

  • 1Department of Digestive Diseases, Wuhan General Hospital of Guangzhou Command PLA, Wuhan, Hubei Province, PR China.

Pathologie-Biologie
|December 15, 2010
PubMed

Insights

Notch1 and Jagged1 proteins are elevated in colon adenocarcinoma, suggesting their involvement in cancer development. This study investigated Notch1 and Jagged1 expression in colon cancer tissues.

Area of Science:

  • Oncology
  • Molecular Biology
  • Gastroenterology

Background:

  • Aberrant Notch pathway signaling is implicated in various cancers.
  • The specific roles of Notch1 and Jagged1 in colon adenocarcinoma remain unclear.

Purpose of the Study:

  • To investigate the expression patterns of Notch1 and Jagged1 in human colon adenocarcinoma.
  • To determine the correlation between Notch1/Jagged1 expression and clinicopathological features of colon cancer.

Main Methods:

  • Immunohistochemistry was used to detect Notch1 and Jagged1 protein expression in 65 colon adenocarcinoma and 60 adjacent nontumor tissues.
  • Western blot and RT-PCR analyzed protein and mRNA levels in 10 paired fresh colon cancer and adjacent nontumor samples.

Main Results:

  • Both Notch1 and Jagged1 were detected in the cytoplasm of neoplastic colon cells.
  • Significantly higher protein and mRNA levels of Notch1 and Jagged1 were observed in colon adenocarcinoma compared to nontumor tissues.
  • Notch1 expression showed a positive correlation with advanced tumor stage.

Conclusions:

  • Notch1 and Jagged1 are upregulated in human colon adenocarcinoma.
  • The Notch1/Jagged1 signaling pathway may contribute to the pathogenesis of colon adenocarcinoma.

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