Related Experiment Videos
[Activity of cefapirin against bacterial strains isolated from acute otitis media in children]
M Simonet1, J L Herrmann, P Gehanno
1Laboratoire de Microbiologie, Hôpital Necker-Enfants Malades, Paris.
Abstract:
The antibacterial activity of cefapirin was tested against 210 strains isolated from the auricular exudate of childrens' acute otitis media. For 112 strains of Haemophilus studied (20% secreted a beta-lactamase), the MIC 50 and 90 of cefapirin were 2 and 4 mg/l respectively. Ten strains of Branhamella catarrhalis were tested (9 secreted a beta-lactamase) and the MIC ranged from 0.25 to 4 mg/l. Against Streptococcus pneumoniae, cefapirin has an extremely high activity with MIC 50 and 90 less than 0.06 mg/l. Of the strains of Staphylococcus aureus sensitive to meticillin, one had a MIC less than 0.06 mg/l, and 11 had a MIC = 0.25 mg/l. For 14 strains of Enterobacteriaceae studied, we obtained a MIC 50 of 8 mg/l and a MIC 90 of 32 mg/l. The results show that cefapirin is an antibiotic particularly suitable for the treatment of acute otitis media.
Insights
Cefapirin demonstrates potent antibacterial activity against key pathogens causing acute otitis media in children. This antibiotic shows particular effectiveness against Streptococcus pneumoniae, making it a promising treatment option.
Area of Science:
- Microbiology
- Pharmacology
- Pediatrics
Background:
- Acute otitis media (AOM) is a common childhood infection.
- Identifying effective antibiotic treatments is crucial for managing AOM.
- Bacterial resistance necessitates ongoing evaluation of antibiotic efficacy.
Purpose of the Study:
- To evaluate the in vitro antibacterial activity of cefapirin.
- To determine the minimum inhibitory concentrations (MICs) of cefapirin against pathogens commonly found in pediatric AOM.
- To assess the suitability of cefapirin for treating AOM.
Main Methods:
- Antibacterial activity testing of cefapirin.
- Isolation of 210 bacterial strains from pediatric AOM auricular exudate.
- Determination of MIC 50 and MIC 90 values for various bacterial species.
Main Results:
- Cefapirin exhibited high activity against Streptococcus pneumoniae (MIC 50/90 < 0.06 mg/L).
- Effective concentrations were observed against Haemophilus strains (MIC 50/90 = 2/4 mg/L) and Branhamella catarrhalis (MIC range 0.25-4 mg/L).
- Moderate activity was noted against Enterobacteriaceae (MIC 50/90 = 8/32 mg/L) and methicillin-susceptible Staphylococcus aureus.
Conclusions:
- Cefapirin displays significant in vitro antibacterial activity against common AOM pathogens.
- The drug's efficacy, particularly against S. pneumoniae, suggests its potential as a treatment for acute otitis media.
- Further clinical studies are warranted to confirm cefapirin's therapeutic benefit in pediatric AOM.