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Related Concept Videos

RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...
Pre-mRNA Processing: RNA Splicing01:32

Pre-mRNA Processing: RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...

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Related Experiment Video

Updated: Jun 6, 2026

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency
08:53

A Reporter Based Cellular Assay for Monitoring Splicing Efficiency

Published on: September 15, 2021

RNA induces conformational changes in the SF1/U2AF65 splicing factor complex.

Ankit Gupta1, Jermaine L Jenkins, Clara L Kielkopf

  • 1Department of Biochemistry and Biophysics, University of Rochester School of Medicine and Dentistry, Rochester, NY 14642, USA.

Journal of Molecular Biology
|December 15, 2010
PubMed
Summary

The study reveals that the SF1/U2AF(65)/RNA complex undergoes a significant conformational change, contracting by 15 Å upon RNA binding. This structural rearrangement is crucial for optimal pre-mRNA splice site positioning in splicing.

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Area of Science:

  • Molecular Biology
  • Structural Biology
  • RNA Biology

Background:

  • Spliceosomes are dynamic ribonucleoprotein complexes essential for pre-mRNA splicing.
  • Splicing factor 1 (SF1) and U2 auxiliary factor (U2AF(65)) are key proteins recognizing the 3' splice site.
  • The conformational dynamics of spliceosome assembly remain poorly understood.

Purpose of the Study:

  • To investigate the structural changes in SF1 and U2AF(65) upon binding to pre-mRNA splice sites.
  • To elucidate the role of RNA in modulating the conformation of splicing factors.

Main Methods:

  • Small-angle X-ray scattering (SAXS) was employed to determine molecular dimensions.
  • Ab initio shape restoration was used to model the molecular envelopes.
  • SAXS data were collected for SF1, U2AF(65), their complex, and their complexes with AdML splice site RNA.

Main Results:

  • The SF1/U2AF(65)/RNA complex showed a substantial contraction (15 Å) in maximum dimension compared to the SF1/U2AF(65) complex without RNA.
  • No significant conformational changes were detected for isolated SF1 or U2AF(65) upon RNA binding.
  • Slight differences in molecular envelope shapes were observed for individual factors and their RNA complexes.

Conclusions:

  • Assembly of the SF1/U2AF(65)/RNA complex induces significant conformational changes.
  • These RNA-induced conformational changes are proposed to optimize the pre-mRNA splice site for subsequent splicing steps.
  • Understanding these dynamics provides insights into the early stages of spliceosome assembly.