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Updated: Jun 6, 2026

Isolation of Murine Lymph Node Stromal Cells

Isolation of Murine Lymph Node Stromal Cells

Published on: August 19, 2014

Lymph node stroma broaden the peripheral tolerance paradigm.

Anne L Fletcher¹, Deepali Malhotra, Shannon J Turley

¹Department of Cancer Immunology and AIDS, Dana Farber Cancer Institute, Boston, USA.

Trends in Immunology

|December 15, 2010

Summary

This summary is machine-generated.

Lymph node stromal cells (LNSC) are key to preventing self-reactive T cells from causing autoimmune disease. These cells induce tolerance to tissue-restricted antigens, a role previously unassigned to non-dendritic cells.

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Area of Science:

Immunology
Cell Biology
Autoimmunity

Background:

Peripheral tolerance mechanisms prevent autoimmune diseases.
Dendritic cells (DCs) have been the primary focus for T cell tolerance induction in lymph nodes.
The role of lymph node stromal cells (LNSC) in tolerance remains less understood.

Purpose of the Study:

To investigate the role of LNSC in inducing deletional tolerance to peripheral tissue-restricted antigens (PTAs).
To determine which LNSC subsets are involved in T cell tolerance.
To broaden the understanding of peripheral tolerance beyond DCs.

Main Methods:

Analysis of LNSC subsets and their interaction with T cells in lymph nodes.
Assessment of T cell tolerance induction by LNSC in steady-state conditions.

Main Results:

LNSC are crucial mediators of deletional tolerance to PTAs.
Fibroblastic reticular cells and lymphatic endothelial cells directly induce tolerance in naive CD8 T cells.
LNSC present PTAs to naive CD8 T cells, a function distinct from DCs.

Conclusions:

LNSC play a significant role in peripheral tolerance by presenting PTAs to naive CD8 T cells.
Peripheral tolerance theory should be expanded to include the contribution of LNSC.
This highlights a novel mechanism for preventing autoimmunity mediated by LNSC.