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Updated: Jun 6, 2026
Two-Step Tag-Free Isolation of Mitochondria for Improved Protein Discovery and Quantification
Published on: June 2, 2023
A novel and effective separation method for single mitochondria analysis
René Pflugradt1, Ulrike Schmidt, Benjamin Landenberger
1Institute of Legal Medicine, Freiburg University Medical Center, Freiburg, Germany. rene.pflugradt@uniklinik-freiburg.de
Abstract:
To investigate the set of mtDNA molecules contained in small biological structures, powerful techniques for separation are required. We tested flow cytometry (FCM(1)), laser capture microdissection (LCM(2)) and a method using optical tweezers (OT(3)) in combination with a 1μ-Ibidi-Slide with regard to their ability to deposit single mitochondrial particles. The success of separation was determined by real-time quantitative PCR (qPCR(4)) and sequencing analysis. OT revealed the highest potential for the separation and deposition of single mitochondrial particles. The study presents a novel setup for effective separation of single mitochondrial particles, which is crucial for the analysis of single mitochondria.
