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Updated: Jun 6, 2026

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria
14:04

Biosensor for Detection of Antibiotic Resistant Staphylococcus Bacteria

Published on: May 8, 2013

MIC versus MBEC to determine the antibiotic sensitivity of Staphylococcus aureus in peritoneal dialysis peritonitis

Louis P Girard1, Howard Ceri, Allan P Gibb

  • 1Division of Nephrology, Department of Medicine, University of Calgary, Calgary, Alberta, Canada. lgirard@ucalgary.ca

Peritoneal Dialysis International : Journal of the International Society for Peritoneal Dialysis
|December 15, 2010
PubMed
Abstract

Insights

Peritoneal dialysis peritonitis caused by Staphylococcus aureus biofilms shows increased antibiotic resistance. Gentamicin retained efficacy against biofilms, unlike other tested antibiotics, suggesting its potential role in treatment.

Area of Science:

  • Microbiology
  • Infectious Diseases
  • Nephrology

Background:

  • Peritoneal dialysis (PD)-related peritonitis is a frequent complication of PD therapy.
  • Bacterial biofilms on PD catheters contribute to recurrent infections and increased antibiotic resistance.
  • Understanding antibiotic efficacy against planktonic versus biofilm bacteria is crucial for effective treatment.

Purpose of the Study:

  • To evaluate the antibiotic susceptibility of Staphylococcus aureus isolates from PD peritonitis in both planktonic and biofilm forms.
  • To compare the effectiveness of standard Minimum Inhibitory Concentration (MIC) testing with Minimum Biofilm Eradication Concentration (MBEC) testing.

Main Methods:

  • Collected 21 clinical isolates of Staphylococcus aureus from patients with PD peritonitis.
  • Assessed antibiotic susceptibility using MIC for planktonic bacteria and MBEC for biofilm bacteria.
  • Employed Chi-square analysis to compare susceptibility results between planktonic and biofilm states.

Main Results:

  • All isolates were susceptible to tested antibiotics in their planktonic form (MIC).
  • Significant loss of antibiotic efficacy was observed in biofilm form (MBEC) for all agents except gentamicin (p < 0.001).
  • Gentamicin demonstrated retained efficacy against Staphylococcus aureus biofilms.

Conclusions:

  • MBEC testing is recommended for persistent, recurrent, or unresponsive PD peritonitis cases to detect potential biofilms.
  • Gentamicin shows promise for empiric gram-negative coverage due to potential synergy against Staphylococcus aureus biofilms.
  • Further evaluation of newer anti-staphylococcal drugs against biofilms using MBEC is warranted.

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