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Updated: Jun 6, 2026

Optical Photothermal Infrared-Fluorescence In Situ Hybridization (OPTIR-FISH)
Published on: February 23, 2024
mathFISH, a web tool that uses thermodynamics-based mathematical models for in silico evaluation of oligonucleotide
L Safak Yilmaz1, Shreyas Parnerkar, Daniel R Noguera
1Department of Civil and Environmental Engineering, University of Wisconsin-Madison, 1415 Engineering Drive, Madison, WI 53706-1691, USA. yilmaz@cae.wisc.edu
Abstract:
Mathematical models of RNA-targeted fluorescence in situ hybridization (FISH) for perfectly matched and mismatched probe/target pairs are organized and automated in web-based mathFISH (http://mathfish.cee.wisc.edu). Offering the users up-to-date knowledge of hybridization thermodynamics within a theoretical framework, mathFISH is expected to maximize the probability of success during oligonucleotide probe design.

