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Updated: Jun 6, 2026

Reconstitution of the Bacterial Glutamate Receptor Channel by Encapsulation of a Cell-Free Expression System
Published on: March 8, 2024
The protein encoded by NCgl1221 in Corynebacterium glutamicum functions as a mechanosensitive channel
Ken-ichi Hashimoto1, Koji Nakamura, Teruo Kuroda
1Department of Green and Sustainable Chemistry, Tokyo Denki University, Tokyo, Japan.
Abstract:
The function of the NCgl1221-encoded protein of Corynebacterium glutamicum was analyzed using Bacillus subtilis as host because a method for preparing the giant provacuole required for electrophysiological studies has been established. Expression of NCgl1221 in a strain deficient in mscL and ykuT, both of which encode mechanosensitive channels, resulted in an 8.9-fold higher cell survival rate upon osmotic downshock than the control. Electrophysiological investigation showed that the giant provacuole prepared from this strain, expressing NCgl1221, exhibited significantly higher pressure-dependent conductance than the control. These findings show that the NCgl1221-encoded protein functions as a mechanosensitive channel.
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