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Related Concept Videos

Tumor Progression02:07

Tumor Progression

Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
Tumor Progression02:07

Tumor Progression

Tumor progression is a phenomenon where the pre-formed tumor acquires successive mutations to become clinically more aggressive and malignant. In the 1950s, Foulds first described the stepwise progression of cancer cells through successive stages.
Colon cancer is one of the best-documented examples of tumor progression. Early mutation in the APC gene in colon cells causes a small growth on the colon wall called a polyp. With time, this polyp grows into a benign, pre-cancerous tumor. Further...
Reporter Genes02:11

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
Commonly used reporter...

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Related Experiment Video

Updated: Jun 6, 2026

Dual Bioluminescence Imaging of Tumor Progression and Angiogenesis
10:56

Dual Bioluminescence Imaging of Tumor Progression and Angiogenesis

Published on: August 1, 2019

Tumor-specific imaging through progression elevated gene-3 promoter-driven gene expression.

Hyo-eun C Bhang1, Kathleen L Gabrielson, John Laterra

  • 1Department of Pharmacology and Molecular Sciences, Johns Hopkins Medical Institutions, Baltimore, Maryland, USA.

Nature Medicine
|December 15, 2010
PubMed
Summary

Researchers developed a new molecular imaging system using the PEG-3 promoter to detect tiny tumors in mouse models. This advance could improve cancer imaging and therapy by tracking gene expression in specific tissues.

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Area of Science:

  • Molecular imaging
  • Oncology
  • Gene expression analysis

Background:

  • Molecular-genetic imaging is transitioning from preclinical research to clinical patient management.
  • Current strategies involve reporter genes and imaging agents to monitor biological processes in vivo.
  • Tissue-specific promoters are crucial for targeted gene expression detection, often requiring amplification.

Purpose of the Study:

  • To evaluate the efficacy of the progression elevated gene-3 (PEG-3) promoter for driving imaging reporters.
  • To assess the PEG-3 promoter's ability to detect micrometastatic disease in cancer models.
  • To explore the potential of PEG-3 promoter-driven systems for cancer imaging and therapy.

Main Methods:

  • Utilized the PEG-3 promoter, derived from a gene associated with tumor progression and metastasis.
  • Drove imaging reporters (bioluminescence and radionuclide-based) selectively using the PEG-3 promoter.
  • Tested the system in mouse models of human melanoma and breast cancer to detect micrometastases.

Main Results:

  • The PEG-3 promoter successfully drove imaging reporters selectively in mouse models.
  • The system enabled the detection of micrometastatic disease in melanoma and breast cancer models.
  • Demonstrated strong promoter activity and tumor specificity.

Conclusions:

  • The PEG-3 promoter is a viable tool for driving imaging reporters for cancer detection.
  • PEG-3 promoter-driven gene expression offers a practical system for facilitating cancer imaging.
  • This approach holds potential for clinical translation in cancer imaging and therapy.