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Sixteen polymorphic simple sequence repeat markers from expressed sequence tags of the Chinese Mitten Crab Eriocheir
Xiang-Gang Gao1, Hong-Jun Li, Yun-Feng Li
1Liaoning Key Laboratory of Marine Fishery Molecular Biology, Liaoning Ocean and Fishery Science Institute, 50 Heishijiao St, Dalian, Liaoning 116023, China; E-Mail: xiangganggao@hotmail.com (X.-G.G.); oceanlhj@163.com (H.-J.L.); yfli2008@yahoo.cn (Y.-F.L.); cnliu51@tom.com (W.-D.L.).
Abstract:
The Chinese mitten crab (Eriocheir sinensis) is an economically important aquaculture species in China. In this study, we developed and evaluated simple sequence repeat markers from expressed sequence tags of E. sinensis. Among the 40 wild E. sinensis individuals tested, 16 loci were polymorphic. The number of alleles per locus ranged from two to ten. The observed heterozygosity ranged from 0.0667 to 0.9667, whereas the expected heterozygosity ranged from 0.0661 to 0.9051. These markers have the potential for use in genetic studies of population structure and intraspecific variation in E. sinensis.
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