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Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview

Ultraviolet and Visible (UV–Vis) Spectroscopy: Overview

Ultraviolet–visible (UV–visible or UV–Vis) spectroscopy is an analytical technique that investigates the interaction between matter and UV–Vis light within the electromagnetic spectrum. This method is widely used for its versatility, simplicity, and relatively quick data acquisition, making it valuable for both qualitative and quantitative analysis. When UV–Vis radiation passes through a material, molecules absorb light depending on the energy required for electronic transitions. As a result...

UV–Vis Spectroscopy of Conjugated Systems

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Organic compounds with conjugated double bonds show strong absorption features in the UV–visible region of the electromagnetic spectrum attributed to π&VeryThinSpace;→&VeryThinSpace;π* electronic excitations. Generally, a UV–vis absorption spectrum is recorded as a plot of absorbance vs wavelength. The wavelength of maximum absorbance, which manifests as a peak in the absorption spectrum, is denoted as λmax.
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UV–Vis Spectroscopy: Woodward–Fieser Rules

UV–Vis Spectroscopy: Woodward–Fieser Rules

UV–Visible absorption spectra of conjugated dienes arise from the lowest energy π&VeryThinSpace;→&VeryThinSpace;π* transitions. The light-absorbing part of the molecule is called the chromophore, and the substituents directly attached to the chromophore are called auxochromes. A strong correlation exists between the absorption maxima, λmax, and the structure of a conjugated π system. The Woodward–Fieser rules predict the value of λmax for a given structure by adding the contributions...

UV–Vis Spectrometers

UV–Vis Spectrometers

The absorbance of UV and visible (UV–visible) radiations is measured using a UV–visible spectrophotometer. Deuterium lamps, which emit UV radiation, and tungsten lamps, which produce radiation in the visible region, are used as light sources in UV–visible spectrophotometers. A monochromator or prism is used for diffraction grating, i.e., to split the incoming radiation into different wavelengths. A system of slits is used to focus the desired wavelength on the sample cell. Samples for...

UV–Vis Spectroscopy: Molecular Electronic Transitions

UV–Vis Spectroscopy: Molecular Electronic Transitions

In Ultraviolet–Visible (UV–Vis) spectroscopy, the absorption of electromagnetic radiation is used to probe the electronic structure of molecules. This technique provides insights into molecular electronic transitions, particularly the movement of electrons between different molecular orbitals. Radiation is absorbed if the energy of the electromagnetic radiation passing through the molecule is precisely equal to the energy difference between the excited and ground states. During this process,...

IR Spectroscopy: Molecular Vibration Overview

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Updated: Jun 6, 2026

Multimodal Nonlinear Hyperspectral Chemical Imaging Using Line-Scanning Vibrational Sum-Frequency Generation Microscopy

Multimodal Nonlinear Hyperspectral Chemical Imaging Using Line-Scanning Vibrational Sum-Frequency Generation Microscopy

Published on: December 1, 2023

Linked Exploratory Visualizations for Uncertain MR Spectroscopy Data.

David Feng, Lester Kwock, Yueh Lee

Visualization and Data Analysis

|December 15, 2010

Summary

This summary is machine-generated.

This study introduces a novel visualization system for magnetic resonance spectroscopy (MRS) data. The system aids radiologists in identifying crucial relationships between brain metabolites and anatomy for improved tumor and lesion diagnosis.

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A Multimodal Wide-Field Fourier-Transform Raman Microscope

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Published on: December 1, 2023

A Multimodal Wide-Field Fourier-Transform Raman Microscope

A Multimodal Wide-Field Fourier-Transform Raman Microscope

Published on: December 30, 2025

Area of Science:

Medical Imaging
Data Visualization
Neuroscience

Background:

Magnetic Resonance Spectroscopy (MRS) generates 3D metabolite concentration fields.
Understanding metabolite-anatomy relationships is key for diagnosing brain tumors and lesions.

Purpose of the Study:

To develop an integrated visualization system for MRS data.
To enhance the identification of relationships between metabolic and anatomical data.

Main Methods:

A linked visualization system comprising Scaled Data-Driven Spheres, augmented parallel coordinates, and a slice plane.
Novel parallel coordinates features: linear function brushing and new axis construction.
Interactions between linked visualizations to explore data.

Main Results:

Users discovered significant relationships between metabolites and brain anatomy.
The system facilitates accurate data value extraction and uncertainty incorporation.
Specialized brushing interactions aid in identifying linear relationships between scalar fields.

Conclusions:

The developed visualization system effectively aids radiologists in analyzing complex MRS data.
The system aids in the diagnosis and treatment of brain tumors and lesions by revealing metabolite-anatomy correlations.
Novel contributions to parallel coordinates enhance the analysis of multidimensional scientific data.