Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Phase Transitions: Melting and Freezing02:39

Phase Transitions: Melting and Freezing

Heating a crystalline solid increases the average energy of its atoms, molecules, or ions, and the solid gets hotter. At some point, the added energy becomes large enough to partially overcome the forces holding the molecules or ions of the solid in their fixed positions, and the solid begins the process of transitioning to the liquid state or melting. At this point, the temperature of the solid stops rising, despite the continual input of heat, and it remains constant until all of the solid is...
Cryo-electron Microscopy01:28

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Microplastic retention within infiltration basins used for managed aquifer recharge: Effectiveness and key controlling factors.

Environmental pollution (Barking, Essex : 1987)·2026
Same author

Microplastics as Emerging Cotracers in Groundwater Quality Assessments.

ACS ES&T water·2026
Same author

Re-evaluating evidence for a 'naming bias' in scientific awards.

Nature human behaviour·2026
Same author

Environmental factors affecting streambed microplastics in a non-perennial river catchment.

Environmental research·2026
Same author

Strategic choices of attack location allow predators to counter a collective prey defence.

Proceedings. Biological sciences·2026
Same author

Effect of hybrid field coupling in nanostructured surfaces on anisotropic signal detection in nanoscale infrared spectroscopic imaging methods.

Faraday discussions·2026

Related Experiment Video

Updated: Jun 6, 2026

A Microfluidic Approach for the Study of Ice and Clathrate Hydrate Crystallization
08:01

A Microfluidic Approach for the Study of Ice and Clathrate Hydrate Crystallization

Published on: August 18, 2022

Freezing single molecule dynamics on interfaces and in polymers.

Stefan Krause1, Pedro F Aramendia, Daniela Täuber

  • 1Institute of Physics and nanoMA (Center for nanostructured Materials and Analysis), Chemnitz University of Technology, 09107, Chemnitz, Germany. stefan.krause@physik.tu-chemnitz.de

Physical Chemistry Chemical Physics : PCCP
|December 15, 2010
PubMed
Summary
This summary is machine-generated.

Single molecule spectroscopy reveals how temperature and polymer environment affect perylene diimide fluorescence. Molecular mobility freezing correlates with spectral diffusion, explained by a two-level system model.

More Related Videos

Synthesis of Cyclic Polymers and Characterization of Their Diffusive Motion in the Melt State at the Single Molecule Level
06:55

Synthesis of Cyclic Polymers and Characterization of Their Diffusive Motion in the Melt State at the Single Molecule Level

Published on: September 26, 2016

Flash-and-Freeze: A Novel Technique to Capture Membrane Dynamics with Electron Microscopy
10:01

Flash-and-Freeze: A Novel Technique to Capture Membrane Dynamics with Electron Microscopy

Published on: May 1, 2017

Related Experiment Videos

Last Updated: Jun 6, 2026

A Microfluidic Approach for the Study of Ice and Clathrate Hydrate Crystallization
08:01

A Microfluidic Approach for the Study of Ice and Clathrate Hydrate Crystallization

Published on: August 18, 2022

Synthesis of Cyclic Polymers and Characterization of Their Diffusive Motion in the Melt State at the Single Molecule Level
06:55

Synthesis of Cyclic Polymers and Characterization of Their Diffusive Motion in the Melt State at the Single Molecule Level

Published on: September 26, 2016

Flash-and-Freeze: A Novel Technique to Capture Membrane Dynamics with Electron Microscopy
10:01

Flash-and-Freeze: A Novel Technique to Capture Membrane Dynamics with Electron Microscopy

Published on: May 1, 2017

Area of Science:

  • Physical Chemistry
  • Spectroscopy
  • Materials Science

Background:

  • Perylene diimide (PDI) molecules exhibit complex fluorescence spectra.
  • Understanding spectral diffusion is crucial for PDI applications.

Purpose of the Study:

  • Investigate heterogeneous line broadening and spectral diffusion in PDI fluorescence.
  • Determine the influence of temperature and polymer environment on PDI spectral dynamics.

Main Methods:

  • Time-dependent single molecule spectroscopy.
  • Controlled temperature variation (surface and PMMA embedding).
  • Two-level system (TLS) modeling for spectral fluctuations.

Main Results:

  • Observed strong correlation between temperature, environment, and spectral diffusion.
  • Tracked freezing of molecular mobility with decreasing temperature.
  • Noted changes in optical transition energies due to conformation and polymer matrix interactions.

Conclusions:

  • Molecular mobility and spectral diffusion are intrinsically linked.
  • The surrounding polymer matrix significantly impacts PDI optical properties.
  • The TLS model effectively explains observed spectral fluctuations in PDI systems.