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Updated: Jun 6, 2026

Evaluating In Vitro DNA Damage Using Comet Assay
Published on: October 11, 2017
[Determination of DNA damage in vitro]
1Lab of Radiobiology & Experimental Radiooncology, University Medical Center Hamburg-Eppendorf, 20246 Hamburg. dikomey@uke.uni-hamburg.de
Abstract:
Ionising irradiation acts primarily via induction of DNA damage, among which double-strand breaks are the most important lesions. These lesions may lead to lethal chromosome aberrations, which are the main reason for cell inactivation. Double-strand breaks can be repaired by several different mechanisms. The regulation of these mechanisms appears be fairly different for normal and tumour cells. Among different cell lines capacity of double-strand break repair varies by only few percents and is known to be determined mostly by genetic factors. Knowledge about double-strand break repair mechanisms and their regulation is important for the optimal application of ionising irradiation in medicine.
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