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Updated: Jun 6, 2026

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
Published on: December 25, 2015
Denitrification of a landfill leachate with high nitrate concentration in an anoxic rotating biological contactor
Susana Cortez1, Pilar Teixeira, Rosário Oliveira
1IBB-Institute for Biotechnology and Bioengineering, Centre of Biological Engineering, University of Minho, Braga, Portugal.
Abstract:
The denitrification performance of a lab-scale anoxic rotating biological contactor (RBC) using landfill leachate with high nitrate concentration was evaluated. Under a carbon to nitrogen ratio (C/N) of 2, the reactor achieved N-NO(3)(-) removal efficiencies above 95% for concentrations up to 100 mg N-NO(3)(-) l(-1). The highest observed denitrification rate was 55 mg N-NO(3)(-) l(-1) h(-1) (15 g N-NO(3)(-) m(-2) d(-1)) at a nitrate concentration of 560 mg N-NO(3)(-) l(-1). Although the reactor has revealed a very good performance in terms of denitrification, effluent chemical oxygen demand (COD) concentrations were still high for direct discharge. The results obtained in a subsequent experiment at constant nitrate concentration (220 mg N-NO(3)(-) l(-1)) and lower C/N ratios (1.2 and 1.5) evidenced that the organic matter present in the leachate was non-biodegradable. A phosphorus concentration of 10 mg P-PO(4)(3-) l(-1) promoted autotrophic denitrification, revealing the importance of phosphorus concentration on biological denitrification processes.
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