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Published on: September 20, 2013
Glycol methacrylate embedding for improved morphological, morphometrical, and immunohistochemical investigations
Hélio Chiarini-Garcia1, Gleydes Gambogi Parreira, Fernanda R C L Almeida
1Laboratory of Structural Biology and Reproduction, Department of Morphology, ICB, Federal University of Minas Gerais, Belo Horizonte, MG, Brazil. chiarini@icb.ufmg.br
Abstract:
Glycol methacrylate (GMA), a water and ethanol miscible plastic resin, is a medium handy to use for light microscopy embedding that has a number of advantages than paraffin embedding. The GMA improves the histological, morphometrical, and immunohistochemical evaluations, mainly due to the accurate assessment of cytological details. This chapter focuses on our experience in the GMA processing and describes in detail the fixation, embedding, and staining methods that we have been using for testes evaluations.
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