Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A NECAP1 Nonsense Variant is Associated With Leukoencephalomyelopathy With Oligodendroglial Dysplasia in a Belgian Malinois With Spinocerebellar Ataxia.

Animal genetics·2026
Same author

Vaginal host-microbe signatures linked to placental outcomes in mares.

Equine veterinary journal·2026
Same author

In ovo versus ex ovo incubation differentially shapes chorioallantoic membrane maturation, angiogenesis, and tumor growth.

Scientific reports·2026
Same author

Computed Tomography, Magnetic Resonance, Cross-Sectional Anatomy, and 3D Volume Rendering of the Nasal Cavity and Paranasal Sinuses of the Alpaca (Vicugna pacos).

Veterinary radiology & ultrasound : the official journal of the American College of Veterinary Radiology and the International Veterinary Radiology Association·2026
Same author

Hemangiosarcoma: A Systematic Review of Biomarkers in Diagnosis, Prognosis, and Therapeutic Strategies.

Veterinary and comparative oncology·2026
Same author

Correction: Collagen composition in equine exuberant granulation tissue reflects tissue immaturity.

PloS one·2026

Related Experiment Video

Updated: Jun 6, 2026

Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue
05:46

Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue

Published on: June 9, 2020

Quantitative mRNA expression analysis in kidney glomeruli using microdissection techniques.

Ward De Spiegelaere1, Pieter Cornillie, Mario Van Poucke

  • 1Department of Morphology, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium. Ward.DeSpiegelaere@UGent.be

Histology and Histopathology
|December 15, 2010
PubMed
Summary

Microdissection techniques enable molecular analysis of specific kidney cells, overcoming tissue heterogeneity. Careful optimization is crucial for RNA integrity and accurate gene expression analysis using RT-qPCR.

More Related Videos

Assessment of Kidney Function in Mouse Models of Glomerular Disease
09:16

Assessment of Kidney Function in Mouse Models of Glomerular Disease

Published on: June 30, 2018

Quantitative Real-Time PCR Evaluation of microRNA Expressions in Mouse Kidney with Unilateral Ureteral Obstruction
07:36

Quantitative Real-Time PCR Evaluation of microRNA Expressions in Mouse Kidney with Unilateral Ureteral Obstruction

Published on: August 27, 2020

Related Experiment Videos

Last Updated: Jun 6, 2026

Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue
05:46

Application of Laser Microdissection to Uncover Regional Transcriptomics in Human Kidney Tissue

Published on: June 9, 2020

Assessment of Kidney Function in Mouse Models of Glomerular Disease
09:16

Assessment of Kidney Function in Mouse Models of Glomerular Disease

Published on: June 30, 2018

Quantitative Real-Time PCR Evaluation of microRNA Expressions in Mouse Kidney with Unilateral Ureteral Obstruction
07:36

Quantitative Real-Time PCR Evaluation of microRNA Expressions in Mouse Kidney with Unilateral Ureteral Obstruction

Published on: August 27, 2020

Area of Science:

  • Nephrology
  • Molecular Biology
  • Genomics

Background:

  • Renal tissue's cellular heterogeneity complicates molecular analysis.
  • Traditional methods like glomerular sieving lack specificity.
  • New tools like RT-qPCR and microarrays require precise sample isolation.

Purpose of the Study:

  • To review microdissection techniques for kidney research.
  • To discuss challenges in molecular analysis of microdissected renal cells.
  • To highlight critical factors for quantitative mRNA analysis.

Main Methods:

  • Literature review of microdissection techniques in kidney research.
  • Discussion of laser-assisted microdissection (LAM).
  • Focus on quantitative mRNA expression analysis via RT-qPCR.

Main Results:

  • Microdissection allows isolation of specific cell populations (e.g., glomerular, tubular).
  • LAM enables molecular analysis of small cell groups from heterogeneous tissue.
  • Drawbacks include small sample size, need for sensitive assays, and potential RNA degradation.

Conclusions:

  • Microdissection techniques are valuable for studying renal disease and development at the cellular level.
  • Optimization and quality control are essential to overcome limitations of microdissection for RT-qPCR.
  • Accurate molecular profiling of specific kidney cell types is achievable with careful methodology.