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Published on: July 17, 2021
[Identification of the new allele D18S1364-10 by sequence analysis]
Ying Zhao1, Wei-hua Xu, Sheng-miao Fu
1Central Laboratory, Hainan Provincial People's Hospital, Haikou, Hainan, P.R. China. zhaoying_2590@sina.com
Objective:
To identify a new allele by analyzing the polymorphism of D18S1364 in Power Plex(TM)16.
Methods:
An abnormal band was found in paternity test by short tandem repeat-PCR, and collected by gel extraction. Then the DNA was amplified, cloned and sequenced.
Results:
A fragment containing eight bases less than the minimal allele in the D18S1364 ladder, was found with the core sequence of (ATCT)₆(ATGT)₁(ATCT)₃. The allele was D18S1364-10.
Conclusion:
The D18S1364-10 allele was found and reported for the first time in China.
