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Flow Cytometry

Flow Cytometry

The development of flow cytometry techniques began in 1934 with initial attempts by Andrew Moldavan, a bacteriologist who counted the cells in a flowing capillary system. Moldavan pumped cells through a capillary tube focused under a microscope for visualization. The invention of photometry allowed the measurement of differentially-stained cells, and Louis Kamentsky developed the first multiparameter flow cytometer in 1965 to identify and count the cancer cells in cervical tissue specimens.
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Updated: Jun 6, 2026

Sensitive Detection of Proteopathic Seeding Activity with FRET Flow Cytometry

Sensitive Detection of Proteopathic Seeding Activity with FRET Flow Cytometry

Published on: December 8, 2015

An efficient and rapid transgenic pollen screening and detection method using flow cytometry.

Hong S Moon¹, Shigetoshi Eda, Arnold M Saxton

¹Department of Plant Sciences, University of Tennessee, Knoxville, TN 37996, USA.

Biotechnology Journal

|December 15, 2010

Summary

This summary is machine-generated.

Flow cytometry offers a rapid and accurate method for quantifying transgenic pollen, crucial for studying gene flow and transgene containment. This technique efficiently detects rare transgenic pollen, overcoming limitations of traditional methods.

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Last Updated: Jun 6, 2026

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Flow Cytometric Analysis of Bimolecular Fluorescence Complementation: A High Throughput Quantitative Method to Study Protein-protein Interaction

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Published on: August 15, 2013

Area of Science:

Agricultural Science
Molecular Biology
Genetics

Background:

Transgenic pollen is a key factor in transgene flow and assessing containment effectiveness.
Current methods like microscopy and progeny analysis are time-consuming and labor-intensive for rare event detection.
A need exists for rapid, high-throughput methods for transgenic pollen analysis.

Purpose of the Study:

To evaluate the accuracy of flow cytometry for quantifying transgenic pollen in practical scenarios.
To assess the method's efficacy with low and high levels of green fluorescent protein (GFP) expression.
To determine the speed and throughput of flow cytometry for pollen analysis.

Main Methods:

A suspension of non-transgenic tobacco pollen was mixed with verified transgenic tobacco pollen.
Transgenic pollen synthesized varying amounts of green fluorescent protein (GFP).
Flow cytometry was used to quantify the spiked transgenic pollen grains.

Main Results:

Flow cytometry detected approximately 75% of pollen with low GFP synthesis and 100% with high GFP synthesis.
The method demonstrated high throughput, capable of analyzing 5000 pollen grains per minute.
The accuracy was validated for detecting rare transgenic pollen events.

Conclusions:

Flow cytometry provides a rapid, accurate, and high-throughput method for transgenic pollen quantification.
This technique is suitable for studying gene flow and assessing transgene containment effectiveness.
The method overcomes the limitations of traditional, laborious pollen analysis techniques.