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Discovery of New Intracellular Pathogens by Amoebal Coculture and Amoebal Enrichment Approaches
Published on: October 27, 2013
Host range of obligate intracellular bacterium Parachlamydia acanthamoebae
Yasuhiro Hayashi1, Shinji Nakamura, Junji Matsuo
1Department of Medical Laboratory Sciences, Hokkaido University Graduate School of Health Sciences, Sapporo, Hokkaido 060-0812, Japan.
Abstract:
The obligate intracellular bacterium Parachlamydia acanthamoebae is a potential human pathogen, but the host range of the bacteria remains unknown. Hence, the growth of P. acanthamoebae Bn₉ in protozoa (Tetrahymena, Acanthamoeba, Dictyostelium) and mammalian cells (HEp-2, Vero, THP-1, PMA-stimulated THP-1, Jurkat) was assessed using an AIU assay which had been previously established by the current authors. P. acanthamoebae grew in Acanthamoeba but not in the other cell types. The growth was also confirmed using DAPI staining, FISH and TEM. These results indicate that the host range of P. acanthamoebae is limited.
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