Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Peptide-Functionalized Liposomal Nanocarriers for Targeted Therapy of Liver Fibrosis and Hepatocellular Carcinoma: Design, Mechanisms, and Clinical Prospects.

ACS pharmacology & translational science·2026
Same author

Rapid Analysis of Pacific Ciguatoxins in Fish Extracts with a Lateral Flow Assay.

Analytical chemistry·2026
Same author

Exploring spirituality in palliative care services: an All-Ireland survey.

BMC palliative care·2026
Same author

Bidirectional regulation of liver sinusoidal clearance by amino acid nanofibers and IGFBP4 complex: effects on HbA1c.

Journal of nanobiotechnology·2025
Same author

Neurobiological Contributions to Speech and Language Interventions: Applications to Developmental Populations.

Developmental neuropsychology·2025
Same author

Quantum-enhanced nanodiamond rapid test advances early SARS-CoV-2 antigen detection in clinical diagnostics.

Nature communications·2025

Related Experiment Video

Updated: Jun 6, 2026

Microfluidic Production of Lysolipid-Containing Temperature-Sensitive Liposomes
09:51

Microfluidic Production of Lysolipid-Containing Temperature-Sensitive Liposomes

Published on: March 3, 2020

Signal-enhancing thermosensitive liposomes for highly sensitive immunosensor development.

Rükan Genç1, Deirdre Murphy, Alex Fragoso

  • 1Nanobiotechnology and Bioanalysis Group, Departament d'Enginyería Química, Universitat Rovira i Virgili, Avinguda Països Catalans, 26, 43007 Tarragona, Spain.

Analytical Chemistry
|December 16, 2010
PubMed
Summary
This summary is machine-generated.

Enzyme-encapsulating liposomes significantly enhance ultrasensitive detection of carcinoembryonic antigen (CEA). This novel amperometric immunosensor achieves detection limits two orders of magnitude lower than traditional methods, showcasing liposomes as powerful signal enhancement tools.

More Related Videos

Antigenic Liposomes for Generation of Disease-specific Antibodies
10:31

Antigenic Liposomes for Generation of Disease-specific Antibodies

Published on: October 25, 2018

Cell-Free Production of Proteoliposomes for Functional Analysis and Antibody Development Targeting Membrane Proteins
08:46

Cell-Free Production of Proteoliposomes for Functional Analysis and Antibody Development Targeting Membrane Proteins

Published on: September 22, 2020

Related Experiment Videos

Last Updated: Jun 6, 2026

Microfluidic Production of Lysolipid-Containing Temperature-Sensitive Liposomes
09:51

Microfluidic Production of Lysolipid-Containing Temperature-Sensitive Liposomes

Published on: March 3, 2020

Antigenic Liposomes for Generation of Disease-specific Antibodies
10:31

Antigenic Liposomes for Generation of Disease-specific Antibodies

Published on: October 25, 2018

Cell-Free Production of Proteoliposomes for Functional Analysis and Antibody Development Targeting Membrane Proteins
08:46

Cell-Free Production of Proteoliposomes for Functional Analysis and Antibody Development Targeting Membrane Proteins

Published on: September 22, 2020

Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Biosensors

Background:

  • Liposomes offer potential as nanovesicles for sensitive detection systems.
  • Their capacity to encapsulate signal-enhancing molecules makes them ideal for improving detection limits.
  • Thermosensitive liposomes are explored for their utility in advanced diagnostic tools.

Purpose of the Study:

  • To develop an ultrasensitive amperometric immunosensor for carcinoembryonic antigen (CEA) detection.
  • To evaluate enzyme-encapsulating thermosensitive liposomes as signal enhancement tools.
  • To compare different bioconjugation methods for antibody attachment to liposomes.

Main Methods:

  • Five bioconjugation methods were assessed for linking anti-CEA antibodies to horseradish peroxidase (HRP)-encapsulating liposomes.
  • ζ-Potential measurements monitored liposome modification success and antibody affinity.
  • Temperature-induced lysis was optimized and compared to detergent-based methods.
  • Biotin-streptavidin and SATA/Sulfo-SMCC chemistries were employed for liposome modification.

Main Results:

  • Temperature-induced liposome lysis proved highly effective.
  • Modified liposomes achieved detection limits two orders of magnitude lower than HRP-antibody conjugates (0.080 ng/mL and 0.0113 ng/mL).
  • Signal amplification of 11-fold and 9-fold was observed with biotin-streptavidin and SATA/Sulfo-SMCC modified liposomes, respectively.

Conclusions:

  • Enzyme-encapsulating thermosensitive liposomes serve as potent signal enhancement tools for immunosensors.
  • The developed immunosensor demonstrates superior sensitivity and lower detection limits for CEA.
  • Liposome-based nanovesicles represent a promising strategy for advancing diagnostic detection systems.