Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Induced Pluripotent Stem Cells01:13

Induced Pluripotent Stem Cells

Stem cells are undifferentiated cells that divide and produce different types of cells. Ordinarily, cells that have differentiated into a specific cell type are post-mitotic—that is, they no longer divide. However, scientists have found a way to reprogram these mature cells so that they “de-differentiate” and return to an unspecialized, proliferative state. These cells are also pluripotent like embryonic stem cells—able to produce all cell types—and are therefore called induced pluripotent stem...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Machine learning approach to analyzing complex care coordination patterns for medically complex children.

BMC medical informatics and decision making·2026
Same author

Sustained efficacy and long-term outcomes of autologous oral mucosal epithelial cell sheet transplantation for pediatric esophageal anastomotic restenosis.

Scientific reports·2026
Same author

The Expression of Placental 17β-Hydroxysteroid Dehydrogenase Genes Is Associated with the Elevation of Active Androgens and Estrogens in Pregnant Women, but Does Not Affect 11-Oxygenated C19 Steroids.

International journal of molecular sciences·2026
Same author

In-utero cell transplantation for hypophosphatasia with gene-edited hESC-derived MSCs in a murine model.

Molecular therapy. Advances·2026
Same author

The feasibility of human embryonic stem cell (hESC)-derived cartilage for tracheoplasty.

Regenerative therapy·2025
Same author

Generation of implant-type tissue-engineered cartilage from human embryonic stem cell-derived chondrocytes.

Regenerative therapy·2025

Related Experiment Video

Updated: Jun 6, 2026

A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening
07:18

A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening

Published on: May 12, 2017

Lectin microarray analysis of pluripotent and multipotent stem cells.

Masashi Toyoda1, Mayu Yamazaki-Inoue, Yoko Itakura

  • 1Department of Reproductive Biology, National Institute for Child Health and Development, 2-10-1 Okura, Setagaya-ku, Tokyo 157-8535, Japan.

Genes to Cells : Devoted to Molecular & Cellular Mechanisms
|December 16, 2010
PubMed
Summary

Lectin microarray analysis effectively distinguishes stem cell types and pluripotency. This glycomic approach offers a novel method for quality control in regenerative medicine and cell-based therapies.

More Related Videos

Kinetic Measurement and Real Time Visualization of Somatic Reprogramming
08:56

Kinetic Measurement and Real Time Visualization of Somatic Reprogramming

Published on: July 30, 2016

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions
09:34

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions

Published on: November 27, 2017

Related Experiment Videos

Last Updated: Jun 6, 2026

A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening
07:18

A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening

Published on: May 12, 2017

Kinetic Measurement and Real Time Visualization of Somatic Reprogramming
08:56

Kinetic Measurement and Real Time Visualization of Somatic Reprogramming

Published on: July 30, 2016

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions
09:34

Reprogramming Primary Amniotic Fluid and Membrane Cells to Pluripotency in Xeno-free Conditions

Published on: November 27, 2017

Area of Science:

  • Stem cell biology
  • Glycomics
  • Developmental biology

Background:

  • Stem cells possess self-renewal and differentiation capabilities.
  • Identifying stem cell types and states is crucial for research and therapy.
  • Current methods for assessing pluripotency can be complex.

Purpose of the Study:

  • To evaluate lectin microarray analysis for categorizing somatic stem cells.
  • To assess the pluripotency of human embryonic stem (hES) and induced pluripotent stem (iPS) cells.
  • To develop a glycomic-based method for stem cell quality control.

Main Methods:

  • Lectin microarray analysis was applied to murine somatic stem cells, hES cells, and iPS cells.
  • Statistical analysis, including false discovery rate, identified key lectins.
  • A discriminant function based on lectin reactivities was developed to assess pluripotency.

Main Results:

  • Lectin microarray successfully categorized murine somatic stem cells by differentiation potential.
  • Undifferentiated hES cells were clearly distinguished from differentiated cells.
  • Three specific lectins (EEL, MAL, PHA(L)) were identified as optimal for distinguishing hES cell states.
  • The developed discriminant function accurately judged stem cell pluripotency.

Conclusions:

  • Glycomic profiling using lectin microarrays provides a robust method for stem cell characterization.
  • This approach enables precise differentiation of pluripotent and somatic stem cells.
  • Lectin microarray analysis represents a novel strategy for quality control in regenerative medicine and cell-based therapies.