Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Electron Microscope Tomography and Single-particle Reconstruction01:07

Electron Microscope Tomography and Single-particle Reconstruction

Transmission electron microscopy (TEM) can be used to determine the 3D structure of biological samples with the help of techniques such as electron microscope tomography and single-particle reconstruction. While single-particle reconstruction can examine macromolecules and macromolecular complexes in vitro conditions only, tomography permits the study of cell components or small cells in vivo.
Electron Tomography
Electron tomography can be performed either in TEM or STEM (scanning transmission...
Atomic Force Microscopy01:08

Atomic Force Microscopy

Atomic force microscopy (AFM) is a type of scanning probe microscopy that can analyze topographic details of various specimens like ceramics, glass, polymers, and biological samples. AFM offers over 1000 times more resolution than the optical imaging system. Images generated from AFM are three-dimensional surface profiles, offering an advantage over the flat, two-dimensional images from other imaging techniques.
The AFM Probe
The probe is regarded as the heart of any AFM setup and comprises the...
Overview of Microscopy Techniques01:22

Overview of Microscopy Techniques

The early pioneers of microscopy opened a window into the invisible world of microorganisms. In 1830, Joseph Jackson Lister created an essentially modern light microscope. The 20th century saw the development of microscopes that leveraged nonvisible light, such as fluorescence microscopy that uses an ultraviolet light source and electron microscopy that uses short-wavelength electron beams. These advances significantly improved magnification, image resolution, and contrast. By comparison, the...
Computed Tomography01:10

Computed Tomography

Tomography refers to imaging by sections. Computed tomography (CT) is a non-invasive imaging technique that uses computers to analyze several cross-sectional X-rays to reveal minute details about structures in the body.
The technique was invented in the 1970s and is based on the principle that as X-rays pass through the body, they are absorbed or reflected at different levels. In the technique, a patient lies on a motorized platform while a computerized axial tomography (CAT) scanner rotates...
¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)01:20

¹³C NMR: Distortionless Enhancement by Polarization Transfer (DEPT)

When proton-coupled carbon-13 spectra are simplified by a broadband proton decoupling technique, structural information about the coupled protons is lost. Distortionless enhancement by polarization transfer (DEPT) is a technique that provides information on the number of hydrogens attached to each carbon in a molecule. While the DEPT experiment utilizes complex pulse sequences, the pulse delay and flip angle are specifically manipulated. The resulting signals have different phases depending on...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Building a comprehensive quality infrastructure in a large radiography practice.

Radiography (London, England : 1995)·2026
Same author

Laboratory Demonstration of Collisionless Blob Formation via Laser-Produced Plasma Self-Focusing.

Physical review letters·2025
Same author

Bayesian approach to automatic mass-spectrum peak identification in atom probe tomography.

Ultramicroscopy·2020
Same author

Atomic-scale age resolution of planetary events.

Nature communications·2017
Same author

Direct observation of individual hydrogen atoms at trapping sites in a ferritic steel.

Science (New York, N.Y.)·2017
Same author

Clustered field evaporation of metallic glasses in atom probe tomography.

Ultramicroscopy·2016

Related Experiment Video

Updated: Jun 6, 2026

Atom Probe Tomography Studies on the Cu(In,Ga)Se2 Grain Boundaries
09:51

Atom Probe Tomography Studies on the Cu(In,Ga)Se2 Grain Boundaries

Published on: April 22, 2013

Improvements in planar feature reconstructions in atom probe tomography.

D J Larson1, B P Geiser, T J Prosa

  • 1Cameca Instruments Inc., Madison, WI, USA. david.larson@ametek.com

Journal of Microscopy
|December 16, 2010
PubMed
Summary

Atom probe tomography reconstruction artifacts arise from assuming a spherical evaporation surface. A variable projection position method is proposed to improve spatial accuracy in atom probe tomography reconstructions.

More Related Videos

Atom Probe Tomography Analysis of Exsolved Mineral Phases
08:14

Atom Probe Tomography Analysis of Exsolved Mineral Phases

Published on: October 25, 2019

Active Probe Atomic Force Microscopy with Quattro-Parallel Cantilever Arrays for High-Throughput Large-Scale Sample Inspection
05:04

Active Probe Atomic Force Microscopy with Quattro-Parallel Cantilever Arrays for High-Throughput Large-Scale Sample Inspection

Published on: June 13, 2023

Related Experiment Videos

Last Updated: Jun 6, 2026

Atom Probe Tomography Studies on the Cu(In,Ga)Se2 Grain Boundaries
09:51

Atom Probe Tomography Studies on the Cu(In,Ga)Se2 Grain Boundaries

Published on: April 22, 2013

Atom Probe Tomography Analysis of Exsolved Mineral Phases
08:14

Atom Probe Tomography Analysis of Exsolved Mineral Phases

Published on: October 25, 2019

Active Probe Atomic Force Microscopy with Quattro-Parallel Cantilever Arrays for High-Throughput Large-Scale Sample Inspection
05:04

Active Probe Atomic Force Microscopy with Quattro-Parallel Cantilever Arrays for High-Throughput Large-Scale Sample Inspection

Published on: June 13, 2023

Area of Science:

  • Materials Science
  • Nanotechnology
  • Surface Science

Background:

  • Standard atom probe tomography (APT) spatial reconstruction methods often introduce artifacts.
  • These artifacts are frequently linked to the assumption of a perfectly spherical evaporation surface during analysis.

Purpose of the Study:

  • To investigate the actual shape of the evaporating surface in APT.
  • To develop and evaluate a novel reconstruction technique to reduce spatial artifacts.
  • To enhance the accuracy of APT data interpretation, especially for complex material structures.

Main Methods:

  • Simulated and experimental field evaporation were used to study surface morphology.
  • A variable point projection position method was implemented in the reconstruction algorithm.
  • The method was tested on simulated datasets and experimental data from model structures.

Main Results:

  • The study confirmed deviations from a perfectly spherical evaporation surface.
  • The variable projection position method demonstrated a reduction in reconstruction artifacts.
  • Initial results show improved accuracy in reconstructing interfaces between materials with different evaporation fields.

Conclusions:

  • The assumption of a spherical evaporation surface in APT is a significant source of reconstruction error.
  • A variable projection position offers a viable strategy to mitigate these artifacts.
  • This technique holds promise for more accurate 3D reconstructions in APT, particularly for heterogeneous materials.