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Assessment of DNA Contamination in RNA Samples Based on Ribosomal DNA
Published on: January 22, 2018
A preliminary overview of the Diaporthales based on large subunit nuclear ribosomal DNA sequences
Lisa A Castlebury1, Amy Y Rossman, Walter J Jaklitsch
1Systematic Botany and Mycology Laboratory, USDA-ARS, 10300 Baltimore Ave., Beltsville, Maryland, USA 20705.
Abstract:
The ascomycete order Diaporthales includes a number of plant pathogenic fungi such as Cryphonectria parasitica, the chestnut blight fungus, as well as many asexually reproducing fungi without known sexual states. Relationships among genera in the Diaporthales were evaluated as a basis for the recognition of families and to provide a taxonomic framework for the asexually reproducing diaporthalean fungi. Phylogenetic relationships were determined based on analyses of large subunit (LSU) nuclear ribosomal DNA (nrDNA) sequences. Within the Diaporthales 82 sequences representing 69 taxa were analyzed. Results suggest the presence of at least six major lineages within the Diaporthales recognized as the Gnomoniaceae sensu stricto, Melanconidaceae sensu stricto, Schizoparme complex including the anamorph genera Coniella and Pilidiella, Cryphonectria-Endothia complex, Valsaceae sensu stricto, and Diaporthaceae sensu stricto. In addition, six teleomorphic and anamorphic taxa fell within the Diaporthales but were not allied with any of the six lineages.
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