Multi-parameter determination of TNFα, PCT and CRP for point-of-care testing

Area of Science:

• Biomedical Engineering
• Analytical Chemistry
• Immunosensing Technology

Background:

• Sepsis and inflammation are critical health concerns requiring timely and accurate diagnosis.
• Current diagnostic methods for inflammation and sepsis biomarkers can be time-consuming and complex.
• Multiplexed detection of biomarkers offers a more comprehensive diagnostic approach.

Purpose of the Study:

• To demonstrate a proof of concept for a compact optical immunosensor.
• To achieve simultaneous determination of three key inflammation and sepsis parameters: TNFα, PCT, and CRP.
• To establish harmonized assay conditions for multiplexed biomarker detection.

Main Methods:

• Development of a compact optical immunosensor platform.
• Utilized immunoassay principles for specific biomarker capture and detection.
• Optimized and harmonized assay conditions for simultaneous measurement of TNFα, PCT, and CRP.
• Generated standard curves to determine assay sensitivity and dynamic range.

Main Results:

• Successful simultaneous detection of TNFα, PCT, and CRP was achieved.
• Established standard curves with test midpoints (IC50) for each biomarker.
• Reported IC50 values: 380 µg L⁻¹ for TNFα, 2300 µg L⁻¹ for PCT, and 2645 µg L⁻¹ for CRP.
• Demonstrated the feasibility of a multi-parameter approach using the developed immunosensor.

Conclusions:

• The compact optical immunosensor is a viable platform for simultaneous multi-parameter determination of inflammation and sepsis biomarkers.
• This technology holds potential for rapid, point-of-care diagnostics in sepsis and inflammatory conditions.
• Further development could lead to improved clinical decision-making through faster and more comprehensive biomarker analysis.