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Related Experiment Videos

Carbon monoxide oxidation by methanogenic bacteria.

L Daniels, G Fuchs, R K Thauer

    Journal of Bacteriology
    |October 1, 1977
    PubMed
    Summary

    Methanogenic bacteria, including Methanobacterium thermoautotrophicum, can metabolize carbon monoxide (CO) as an energy source. This process involves CO disproportionation and the activity of a CO-dehydrogenating enzyme, though growth on CO is limited.

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    Area of Science:

    • Microbiology
    • Biochemistry
    • Environmental Science

    Background:

    • Methanogenic archaea typically utilize H(2)/CO(2) for growth.
    • Some methanogens demonstrate the ability to consume carbon monoxide (CO) from their environment.

    Purpose of the Study:

    • To investigate the capacity of methanogenic bacteria to utilize CO as a sole energy source.
    • To characterize the CO-dehydrogenating enzyme in Methanobacterium thermoautotrophicum.

    Main Methods:

    • Cultivation of methanogenic bacteria on CO.
    • Enzymatic assays of cell-free extracts to determine hydrogenase and CO-dehydrogenating enzyme activity.
    • Characterization of the CO-dehydrogenating enzyme's properties (pH, temperature, substrate affinity, inhibitors).

    Main Results:

    • Methanobacterium thermoautotrophicum can grow on CO via disproportionation (4CO + 2H(2)O → CH(4) + 3CO(2)), but with significantly reduced growth rates compared to H(2)/CO(2).
    • A CO-dehydrogenating enzyme was identified in M. thermoautotrophicum, utilizing factor 420 (F(420)) as an electron acceptor.
    • Enzyme properties include a high K(m) for CO, optimal activity at higher pH, and sensitivity to cyanide and oxygen.

    Conclusions:

    • Methanogens possess the enzymatic machinery to utilize CO, although it's not an optimal growth substrate.
    • The CO-dehydrogenating enzyme in M. thermoautotrophicum is distinct from that in Clostridium pasteurianum due to its F(420) dependency.

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